Biotinylated Human Transferrin R/CD71 Protein N-His-Avi Tag | Syd Labs BP004123
(悉得）Syd Labs 货号：BP004123 Biotinylated Human Transferrin R/CD71 Protein with the N-His-Avi Tag (生物素化人转铁蛋白 R/CD71 蛋白 N-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.
产品参数
| 货号 | BP004123 |
|---|---|
| 产品名称 | Biotinylated Human Transferrin R/CD71 Protein N-His-Avi Tag | Syd Labs BP004123 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Human |
| 来源 | The recombinant Biotinylated Human Transferrin R/CD71 Protein, (amino acids Cys89-Phe760) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells. |
| Accession | P02786 |
| 产品标签 | N-His-Avi |
| 生物素化 | yes |
| 确定序列 | Cys89-Phe760 |
| 分子量 | The recombinant Biotinylated Human Transferrin R/CD71 protein with the His-Avi tag at the N-terminus has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 80-85 kDa based on Bis-Tris PAGE result. |
| 活性 | Immobilized recombinant Biotinylated Human Transferrin R protein with the N-His-Avi tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Anti-Transferrin R Antibody protein with the N-hFc tag with the EC50 of 4.8 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Biotinylated Human Transferrin R/CD71 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Biotinylated Human Transferrin R/CD71 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Biotinylated Human Transferrin R/CD71 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004123: Biotinylated Human Transferrin R/CD71 Protein with the N-His-Avi Tag (生物素化人转铁蛋白 R/CD71 蛋白 N-His-Avi 标签)
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
请记住我们的产品信息: 生物素化人转铁蛋白 R/CD71 蛋白 （N-His-Avi 标签）BP004123 Syd Labs（Biotinylated Human Transferrin R/CD71 Protein with the N-His-Avi Tag）.