Biotinylated Human LRRC15/LIB Protein C-His-Avi Tag | Syd Labs BP005183
Syd Labs 货号:BP005183 Biotinylated Human LRRC15/LIB Protein with the C-His-Avi Tag (生物素化人 LRRC15/LIB 蛋白 C-His-Avi 标签) is expressed from CHO with His tag and Avi tag at the C-Terminus.It contains Tyr22-Gly538.
产品参数
| 货号 | BP005183 |
|---|---|
| 产品名称 | Biotinylated Human LRRC15/LIB Protein C-His-Avi Tag | Syd Labs BP005183 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | LIB; LRRC15 |
| 物种 | Human |
| 来源 | The recombinant Biotinylated Human LRRC15/LIB Protein, (amino acids Tyr22-Gly538) fused with a His-Avi tag at the C-terminus, was produced in CHO cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | yes |
| 确定序列 | Tyr22-Gly538 |
| 分子量 | The recombinant Biotinylated Human LRRC15/LIB protein with the His-Avi tag at the C-terminus has a predicted MW of 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Anti-LRRC15 Antibody protein with the C-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human LRRC15 protein with the C-His tag with the EC50 of 68.3 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Biotinylated Human LRRC15/LIB Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Biotinylated Human LRRC15/LIB Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Biotinylated Human LRRC15/LIB Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品,不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题,欢迎随时联系Syd Labs国内市场推广合作伙伴:武汉多找找科技有限公司,企业微信:duozhaozhao2024 联系电话:18162581039(龙经理) |
文献
BP005183: Biotinylated Human LRRC15/LIB Protein with the C-His-Avi Tag (生物素化人 LRRC15/LIB 蛋白 C-His-Avi 标签)
The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation. LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.
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