Biotinylated Human GPA33/A33 Protein C-His-Avi Tag | Syd Labs BP005660
(悉得)Syd Labs 货号:BP005660 Biotinylated Human GPA33/A33 Protein with the C-His-Avi Tag (生物素化人 GPA33/A33 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile22-Val235.
产品参数
| 货号 | BP005660 |
|---|---|
| 产品名称 | Biotinylated Human GPA33/A33 Protein C-His-Avi Tag | Syd Labs BP005660 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Glycoprotein A33; GPA33; A33; MGC129986; MGC129987 |
| 物种 | Human |
| 来源 | The recombinant Biotinylated Human GPA33/A33 Protein, (amino acids Ile22-Val235) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | yes |
| 确定序列 | Ile22-Val235 |
| 分子量 | The recombinant Biotinylated Human GPA33/A33 protein with the His-Avi tag at the C-terminus has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Anti-GPA33 Antibody protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GPA33 protein with the C-His tag with the EC50 of 33.2 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Biotinylated Human GPA33/A33 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Biotinylated Human GPA33/A33 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Biotinylated Human GPA33/A33 Proteinn performance should be determined experimentally by the investigator. |
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文献
BP005660: Biotinylated Human GPA33/A33 Protein with the C-His-Avi Tag (生物素化人 GPA33/A33 蛋白 C-His-Avi 标签)
Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
请记住我们的产品信息: 生物素化人 GPA33/A33 蛋白 (C-His-Avi 标签)BP005660 Syd Labs(Biotinylated Human GPA33/A33 Protein with the C-His-Avi Tag).






