AAV5 Titration ELISA Kit

描述

EK0401: AAV5 Titration ELISA Kit

Background

Application: for the quantitative determination of full and empty AAV5 capsids, including intact AAV5 Wild Type virions, AAV5 recombinant virions, assembled AAV5 virions, intact empty AAV5 capsids.
This assay is specific for AAV5 capsids.

Detection range: 3.1^7 capsids/mL – 2.0^9 capsids/mL
Sensitivity: 1.5^7 capsids/mL
Precision: CV < 10% Kit components for the 96-well plate ELISA kit for full and empty AAV5 capsids (1x 96-well microplate): 1) Pre-coated Microplate: 96 well polystyrene microplates coated with monoclonal antibody specific for AAV5 capsids; 1x 96-well microplate 2) Detection Antibody: Biotinylated anti-AAV5 antibody which binds to AAV5 in standard and test samples; 1 vial (30 ul) 3) HRP Conjugate: Streptavidin Peroxidase conjugate that binds to detection antibody and catalyzes color development; 1 bottle (15 ml) 4) AAV5 Capsid Standard: Lyophilized AAV5 powder ; 2 vials (6.5E+08 capsids/vial) 5) 10X Assay Buffer: for diluting standards or samples. Dilute to 1X before use; 1 bottle (10 ml) 6) 20X Wash Buffer: containing PBS and Tween-20. Dilute to 1X before use; 2 bottles (15 ml each) 7) Color Reagent: tetramethylbenzidine (TMB); 1 bottle (15 ml) 8) Stop Solution: 0.5 N hydrochloric acid solution; 1 bottle (10 ml) 9) Product Insert: product description, assay protocol, material data sheet; 1 book. Empty AAV5 capsids lacking the transgene of interest cause unwanted immune response without providing the therapeutic benefit of the transgene. Alongside DNA and infectious titer, determination of the capsid titer is crucial for validating the efficacy and safety of your AAV gene therapy. Our titration kit detects full and empty AAV5 capsids. The AAV5 titration ELISA kit uses sandwich ELISA to determine the titer of AAV5 capsids in the test sample. The capture AAV5 monoclonal antibody is pre-coated and treated on a 96-well reaction plate. AAV5 standard or sample is added to the pre-coated plate and specifically binds to the capture antibody. The biotinylated detection AAV5 antibody is then added to the plate to bind the immune complex. Next, the streptavidin HRP conjugate is added to react with the biotin molecules. The addition of TMB results in color changes and the amplitude of the color change is proportional to the amount of AAV5 capsids that specifically bind to the plate. The reaction is stopped with the addition of stop solution and the absorbance is measured at 450nm. The sample AAV5 capsid titer is calculated from the AAV5 standards titration curve. AAV Titration ELISA Kits:
AAV2 Titration ELISA Kit
AAV5 Titration ELISA Kit
AAV8 Titration ELISA Kit
AAV9 Titration ELISA Kit

Nuclease:
Recombinant Dr. Nuclease
RNase A Enzyme

Please remember our product information: AAV5 Titration ELISA Kit: EK0401 Syd Labs