AAV9 Titration ELISA Kit

描述

EK0400: AAV9 Titration ELISA Kit

Background

Application: for the quantitative determination of full and empty AAV9 capsids, including intact AAV9 Wild Type virions, AAV9 recombinant virions, assembled AAV9 virions, intact empty AAV9 capsids.
This assay is specific for AAV9 capsids.

Detection range: 1.0^7 capsids/mL – 6.5^8 capsids/mL
Sensitivity: 1.0^7 capsids/mL
Precision: CV < 10% Kit components for the 96-well plate ELISA kit for full and empty AAV9 capsids (1x 96-well microplate):
1) Pre-coated Microplate: 96 well polystyrene microplates coated with monoclonal antibody specific for AAV9 capsids; 1x 96-well microplate
2) Detection Antibody: Biotinylated anti-AAV9 antibody which binds to AAV9 in standard and test samples; 1 vial (30 ul)
3) HRP Conjugate: Streptavidin Peroxidase conjugate that binds to detection antibody and catalyzes color development; 1 bottle (15 ml)
4) AAV9 Capsid Standard: Lyophilized AAV9 powder ; 2 vials (6.5E+08 capsids/vial)
5) 10X Assay Buffer: for diluting standards or samples. Dilute to 1X before use; 1 bottle (10 ml)
6) 20X Wash Buffer: containing PBS and Tween-20. Dilute to 1X before use; 2 bottles (15 ml each)
7) Color Reagent: tetramethylbenzidine (TMB); 1 bottle (15 ml)
8) Stop Solution: 0.5 N hydrochloric acid solution; 1 bottle (10 ml)
9) Product Insert: product description, assay protocol, material data sheet; 1 book.

Empty AAV9 capsids lacking the transgene of interest cause unwanted immune response without providing the therapeutic benefit of the transgene. Alongside DNA and infectious titer, determination of the capsid titer is crucial for validating the efficacy and safety of your AAV gene therapy. Our titration kit detects full and empty AAV9 capsids.

The AAV9 titration ELISA kit uses sandwich ELISA to determine the titer of AAV9 capsids in the test sample. The capture AAV9 monoclonal antibody is pre-coated and treated on a 96-well reaction plate. AAV9 standard or sample is added to the pre-coated plate and specifically binds to the capture antibody. The biotinylated detection AAV9 antibody is then added to the plate to bind the immune complex. Next, the streptavidin HRP conjugate is added to react with the biotin molecules. The addition of TMB results in color changes and the amplitude of the color change is proportional to the amount of AAV9 capsids that specifically bind to the plate. The reaction is stopped with the addition of stop solution and the absorbance is measured at 450nm. The sample AAV9 capsid titer is calculated from the AAV9 standards titration curve.

AAV Titration ELISA Kits:
AAV2 Titration ELISA Kit
AAV5 Titration ELISA Kit
AAV8 Titration ELISA Kit
AAV9 Titration ELISA Kit

Nuclease:
Recombinant Dr. Nuclease
RNase A Enzyme

Please remember our product information: AAV9 Titration ELISA Kit: EK0400 Syd Labs