Syd Labs D-荧光素钾盐的作用原理如何?看完这篇就都懂了
Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt,CAS号:115144-35-9)的作用原理如何?Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt,货号MB000102-R70170或MB102-R70170),这款优质产品由美国Syd Labs公司自主研发,在国外众多高校实验室的动物体内实验中展现了卓越性能,并经受了国际市场严格考验。Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt)现正式登陆国内市场,旨在为中国高校的动物实验中心和实验室提供一流服务与品质保证。
Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt,货号:MB000102-R70170或MB102-R70170)因其卓越表现,在欧美多所知名大学的core facilities中获得高度认可,也成为了许多寻求高质量实验材料的研究人员的理想之选。Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt)的优质成效不仅体现在实际应用中,更在众多国际权威学术期刊中得以体现。Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt)被以下两篇文献引用:
16.
Mariko Kamiya, Makoto Matsumoto, Kazuma Yamashita, Tatsunori Izumi, Maho Kawaguchi, Shusaku Mizukami, Masako Tsurumaru, Hidefumi Mukai, Shigeru Kawakami
Pharmaceutics. 2022 Oct 31;14(11):2357. doi: 10.3390/pharmaceutics14112357.
The mice were shaved to reduce photon scattering and absorption by fur. Fifty microliters of mRNA-LNPs were injected intramuscularly. Mice were intraperitoneally injected with 150 μL D-luciferin solution (30 mg/mL, Syd Labs, Boston, MA, USA) in PBS, and in vivo bioluminescent imaging was performed at 10 min of D-luciferin administration using an exposure time of a minute with an IVIS Lumina II (Caliper Life Sciences, Hopkinton, MA, USA) for three consecutive days. The total flux in the region of interest (ROI) was determined using the Living Image 3.0 software (Caliper Life Sciences).
17.
Sayuri Nakamae, Satoshi Miyagawa, Koki Ogawa, Mariko Kamiya, Mayumi Taniguchi, Akari Ono, Maho Kawaguchi, Awet Alem Teklemichael, Jiun-Yu Jian, Tamasa Araki, Yukimi Katagami, Hidefumi Mukai, Takeshi Annoura, Katsuyuki Yui, Kenji Hirayama, Shigeru Kawakami, Shusaku Mizukami
Front Immunol. 2023 Aug 23:14:1116299. doi: 10.3389/fimmu.2023.1116299. eCollection 2023.
To assess the changes in the FLuc mRNA-LNPs over time, BALB/c mice were inoculated with FLuc mRNA-LNPs (5 μg) intravenously, intramuscularly, or subcutaneously. To detect luminescence, the mice were administered 150 mg/kg of D-luciferin (Syd labs, Hopkinton, MA, USA) intraperitoneally and anaesthetized with a mixture of oxygen and isoflurane (Wako, Osaka, Japan). Ten minutes after the administration of D-luciferin, the mice were imaged using IVIS Lumina II (Caliper Life Sciences, Waltham, MA, USA) with an exposure time of 5 s. To detect the tissue distribution of the FLuc mRNA-LNPs, B6 mice were intravenously inoculated with FLuc mRNA-LNPs (5 μg) and administered D-luciferin (150 mg/kg) intraperitoneally 3 h later. The reaction was observed for 10 min. The brains, hearts, livers, spleens, lungs, kidneys, and intestines were collected immediately and imaged using an IVIS imager for 5 s. Bioluminescent signals in the regions of interest were quantified using Living Image 3.0 (Caliper Life Sciences).

品牌名称:Syd Labs
产品名称:D-荧光素钾盐(D-Luciferin Potassium Salt)
货号:MB000102-R70170或MB102-R70170
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