Syd Labs D-荧光素钾盐怎么用于活体成像分析

Syd Labs D-荧光素钾盐怎么用于活体成像分析？这些文献里隐藏着答案

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）怎么用于活体成像分析？Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号MB000102-R70170或MB102-R70170），这款优质产品由美国Syd Labs公司自主研发，在国外众多高校实验室的动物体内实验中展现了卓越性能，并经受了国际市场严格考验。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）现正式登陆国内市场，旨在为中国高校的动物实验中心提供一流服务与品质保证。

Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170)，凭借其卓越性能，获得了欧美众多大学core facilities的高度认可。正因如此，Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt)成为了许多国际知名学术期刊中的实验研究材料。

下面我们一起来看看涉及Syd Labs D-luciferin potassium salt(CAS号：115144-35-9) 的两篇应用文献，看看在具体的实验中如何使用D-荧光素钾盐：

26.

synNotch-programmed iPSC-derived NK cells usurp TIGIT and CD73 activities for glioblastoma therapy

Kyle B Lupo, Xue Yao, Shambhavi Borde, Jiao Wang, Sandra Torregrosa-Allen, Bennett D Elzey, Sagar Utturkar, Nadia A Lanman, MacKenzie McIntosh, Sandro Matosevic

Nat Commun. 2024 Mar 1;15(1):1909. doi: 10.1038/s41467-024-46343-3.

Intracranial xenograft model: Orthotopic patient-derived GBM xenograft models were generated using eighteen 8 week old male NSG (Strain #: 005557) mice as previously described15. Briefly, GBM43-Luc cells (1 × 105) were stereotactically implanted in the right forebrain via a burr hole created using a stereotactic delivery system at a depth of 3.5 mm. After 9 days, mice were randomly assigned to three treatment groups: PBS (n = 6), WT iNK cells (n = 6), and TIGIT.SynNotch.aCD73 iNK cells (n = 6). Mice received weekly intracranial injections of 5 uL of either PBS or NK cells (suspended in 1% IgG-free BSA in PBS) for 3 weeks, and bioluminescence and bodyweight was measured biweekly until the mice reached a predetermined endpoint. D-Luciferin potassium salt was purchased from Syd Labs. Following the study, mouse tumors were harvested for histological analysis. Quantification of histological analysis was performed on four random intratumoral sections from each treatment group at 200x magnification. For NKp46 and Granzyme B, stained cells were counted for each frame. For CD73 and CD155, images were given a score for based on the following formula: 0 = 0% positive staining, 1 = 1–40% positive staining, 2 = 41–75% positive staining, 3 = 75–100% positive staining; 1 = weak staining, 2 = moderate staining, 3 = strong staining. The overall IHC score was calculated by multiplying each staining score. IHC array immunofluorescent staining was scored for each core using the same formula for both CD155 and CD73 expression. In a separate study, GBM43-Luc cells (~8 × 104) were similarly stereotactically implanted in the right forebrain of five 8 week old male NSG mice. After 16 days, mice treated with intravenous (i.v.) injection of 5 × 106 primary NK cells. After 24 h, the mice were sacrificed and whole brain tissues from each mouse were harvested. The presence of NK cells was determined with histological (IHC) analyses by using anti-NKp46 antibody staining, as described.

27.

Development of highly effective anti-mesothelin hYP218 chimeric antigen receptor T cells with increased tumor infiltration and persistence for treating solid tumors

Sakshi Tomar, Jingli Zhang, Manakamana Khanal, Jessica Hong, Abhilash Venugopalan, Qun Jiang, Manjistha Sengupta, Markku Miettinen, Nan Li, Ira Pastan, Mitchell Ho, Raffit Hassan

Mol Cancer Ther. 2022 Jul 5;21(7):1195-1206. doi: 10.1158/1535-7163.MCT-22-0073.

Three different human xenograft models were used to assess the in vivo antitumor efficacy of CAR T cells, including luciferase-expressing OVCAR-8 (ovarian cancer), luciferase-expressing KLM-1 (pancreatic cancer), and NCI-Meso63 (mesothelioma patient-derived primary tumor). Briefly, 5 × 106 OVCAR-8 tumor cells or 3 × 106 KLM-1 tumor cells were implanted i.p., while 1 × 107 NCI-Meso63 non–luciferase-expressing mesothelioma cells were implanted subcutaneously into 5- to 6-week-old female NSG mice. For luciferase-expressing OVCAR-8 and KLM-1 tumor models, tumor growth was monitored weekly via bioluminescence imaging (PerkinElmer, IVIS Lumina III In vivo Imaging System) after intraperitoneal injection of the substrate d-luciferin (Syd Labs, catalog No. MB000102-R70170). NCI-Meso63 subcutaneous tumors were measured weekly using calipers, and the tumor volume was calculated using the formula: tumor volume = length × (width)2 × 0.4. A single intravenous infusion of 1 × 107 CAR T cells was administered either 2 weeks post-tumor implantation in the OVCAR-8 and KLM-1 tumor–bearing mice, or when tumor volume reached ∼180 mm3 for NCI-Meso63 tumor–bearing mice.