Syd Labs D-荧光素钾盐体内实验注意事项有哪些？

Syd Labs D-荧光素钾盐体内实验注意事项有哪些？这些文献带你了解

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，CAS号:115144-35-9）体内实验注意事项有哪些？Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号MB000102-R70170或MB102-R70170），这款久经全球市场验证、由美国Syd Labs公司自主研发的优质产品主要被用于动物体内实验。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）无论是价格还是质量都具有很强的竞争优势，使其在海外市场赢得了广泛赞誉。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）现正式进军中国，旨在为中国高校动物实验中心提供全新的科研动力。

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170）凭借其卓越性能，获得了欧美多所知名大学core facilities的高度评价。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）不仅在众多欧美实验室中被广泛用于动物体内实验，而且还成为了许多寻求高质量实验材料的研究人员的理想之选，并被这些专家学者使用到国际知名学术期刊之中。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）在以下三篇文献中得到了具体应用，彰显了其在生物科学领域的实用价值：

18.

Targeting CA-125 Transcription by Development of a Conditionally Replicative Adenovirus for Ovarian Cancer Treatment

Er Yue, Guangchao Yang, Yuanfei Yao, Guangyu Wang, Atish Mohanty, Fang Fan, Ling Zhao, Yanqiao Zhang, Tamara Mirzapoiazova, Tonya C Walser, Lorna Rodriguez-Rodriguez, Yuman Fong, Ravi Salgia, Edward Wenge Wang

Cancers (Basel). 2021 Aug 24;13(17):4265. doi: 10.3390/cancers13174265.

ID8 cells labeled with a stable firefly luciferase gene were plated in 96-well plates(5000 cell/well) and cultured overnight. Ad5/MUC16-1040/TK-EGFP were added at a multiplicity of infection (MOI) of 20:1. Ganciclovir (GCV; Sigma-Aldrich, St. Louis, MO,USA) was then added to the cells at a final concentration of 10 µM and incubated for an additional 3 days. D-luciferin (potassium salt; Syd Labs, Hopkinton, MA, USA) was added to each well at a final concentration of 100 µg/mL. Luminescence intensity was measured by a Tecan Spark 10M multimode microplate reader.

19.

Pulmonary administration of a CSF-1R inhibitor alters the balance of tumor-associated macrophages and supports first-line chemotherapy in a lung cancer model

Hanming Zhang, Rashed M Almuqbil, Sulaiman S Alhudaithi, Fatemah S Sunbul, Sandro R P da Rocha

Int J Pharm. 2021 Apr 1:598:120350. doi: 10.1016/j.ijpharm.2021.120350. Epub 2021 Feb 2.

The wild type Lewis Lung Carcinoma (wt-LLC) cell line was a gift from the Bos Lab, Department of Pathology, Virginia Commonwealth University (VCU), VA, USA. Wt-LLC cells were transformed in our lab-oratories to express tdTomato (tdT) fluorescent and firefly luciferase (luc) proteins – luc-tdT-LLC (unpublished data). Lentivirus and poly-brene® were gifts from the Koblinski Lab, Department of Pathology,Virginia Commonwealth University (VCU), VA, USA. Trypsin EDTA 1x,Dulbecco’s Modified Eagle’s Medium (DMEM) and puromycin were purchased from Corning Life Science (Durham, NC). Fetal Bovine Serum (FBS) was purchased from Atlanta Biologicals (Minneapolis, MN). RPMI Medium 1640 (GibcoTM) was purchased from Thermo Fisher (Waltham,MA). Pen-Strep was obtained from Invitrogen (Karlsruhe, Germany).Culture plates (96-well) were purchased from Thomas Scientific (Swe-desboro, NJ). D-Luciferin potassium salt was obtained from Syd labs(Southborough, MA). Cisplatin (CIS, purity 99%) was purchased from AvaChem Scientific (San Antonio, TX) and PLX was purchased from Chemgood (Glen Allen, VA). MTT (Thiazolyl Blue Tetrazolium Bromide) was purchased from Research Products International (Mount Prospect,IL). Isoflurane, USP was obtained from Covetrus (Portland, ME).Optimal cutting temperature (OCT) compound was obtained from Sakura Finetek (Torrance, CA) and positively charged glass slides were purchased from Globe Scientific (Mahwah, NJ). Brilliant Violet 421TM anti-mouse F4/80 antibody (catalog# 123131), FITC anti-mouse CD80 antibody (catalog# 104705), and Alexa Fluor® 488 anti-mouse CD206 antibody (catalog# 141709) were obtained from BioLegend (San Diego,CA).

20.

The pro-tumor effect of CD200 expression is not mimicked by agonistic CD200R antibodies

Zofia Pilch, Katarzyna Tonecka, Marcin Skorzynski, Zuzanna Sas, Agata Braniewska, Tomasz Kryczka, Louis Boon, Jakub Golab, Linde Meyaard, Tomasz P Rygiel

PLoS One. 2019 Jan 17;14(1):e0210796. doi: 10.1371/journal.pone.0210796. eCollection 2019.

B16F10 melanoma with or without stable expression of CD200, together with luciferase marker were used (B16F10-EV and B16F10-CD200 respectively). Cd200-/- mice and wild type controls were injected on the right flank with a mixture of B16F10 cells (1.5 × 105) in 30 μl PBS/Matrigel mixture. Melanoma tumor growth was monitored by in vivo bioluminescence imaging. To that end, mice were injected (i.p.) with D-luciferin (Syd Labs, Natick, MA, USA) (150 mg/kg), and after 5 min were anesthetized with isoflurane and visualized using the IVIS Imaging System (Xenogen, Alameda, CA, USA). Images were analyzed with the Living Image 4.3 software package (Caliper Life Science, Hopkinton, MA, USA). To quantify the bioluminescence (BLI) signal of mice, the regions of interest (ROIs) were drawn on the tumor region and results were used to generate growth curves of B16F10 melanoma. Bioluminescence data were presented as average radiance (photons/sec/cm2/steradian).