Syd Labs D-荧光素钾盐适合哪些实验?这些文献有所体现
Syd Labs D-荧光素钾盐适合哪些实验?Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt,货号MB000102-R70170或MB102-R70170),作为一项经过国际市场检验、主要用于动物体内实验的产品,在品质与成本效益上具有双重优势,这表明其在国际市场上具有较大的竞争力。Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt)现已强势登陆中国市场,期待为中国高校的动物实验中心和实验室注入新的活力。
Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt,货号:MB000102-R70170或MB102-R70170),凭借其卓越表现,已赢得欧美众多大学core facilities的一致肯定。Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt)作为美国Syd Labs公司的特色产品之一,不仅在美国及欧洲的实验室中被广泛应用于动物体内实验,还在国际知名的学术期刊中频繁被引用,这有力地证明了Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt)在科研领域的实用价值。
Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt)在以下三篇文献中的部分展现了其在生物科学具体研究领域的广泛适用性和实用性:
13.
Ping-Chih Hsu, Jinbai Miao, Zhen Huang, Yi-Lin Yang, Zhidong Xu, Joanna You, Yuyuan Dai, Che-Chung Yeh, Geraldine Chan, Shu Liu, Anatoly Urisman, Cheng-Ta Yang, David M Jablons, Liang You
Trop Med Health. 2023 Mar 1;51(1):12. doi: 10.1186/s41182-023-00502-2.
Bioluminescent imaging (Caliper Life Sciences, Waltham, MA) was used to monitor in vivo metastasis. D-luciferin potassium salt (Syd Labs, Natick, MA) was injected intraperitoneally into the mice at a dose of 150 mg/kg, and mice were anaesthetized by inhalant isoflurane 1%-5% in oxygen. After 10 minutes, the mice were placed into the Xenogen IVIS spectrum imaging system. Images were recorded with an exposure time of 1 minute. Survival was measured from the day of H2030-BrM3 cell injection to the day mice were killed.
14.
Brandon M Hall, Vitaly Balan, Anatoli S Gleiberman, Evguenia Strom, Peter Krasnov, Lauren P Virtuoso, Elena Rydkina, Slavoljub Vujcic, Karina Balan, Ilya I Gitlin, Katerina I Leonova, Camila R Consiglio, Sandra O Gollnick, Olga B Chernova, Andrei V Gudkov
Comparative Study Aging (Albany NY). 2017 Aug 2;9(8):1867-1884. doi: 10.18632/aging.101268.
In vivo bioluminescence from the abdomen of mice was performed as previously described [23]. Briefly, mice were injected intraperitoneally with a 200 μl solution of 15 mg/mL D-luciferin potassium salt (Syd Labs; Boston, MA) in D-PBS without calcium and magnesium. At 10 minutes post-injection, isoflurane-anesthetized mice were placed into the IVIS Spectrum imaging system (PerkinElmer; Waltham, MA) for detection of luciferase activity (120-second exposure). Bioluminescence in p16Ink4a/Luc mice was quantified as total flux (p/s) of luminescent signal from the abdomen using via Living Image® software (PerkinElmer). For imaging of individual organs ex vivo, mice were perfused with 20 mL of D-luciferin in D-PBS (300 μg/mL). The organs were quickly excised, rinsed in D-PBS, and placed in the perfusion solution prior to positioning on a dry surface for IVIS imaging (120-second exposure).
15.
Visualizing CAR-T cell Immunotherapy Using 3 Tesla Fluorine-19 MRI
Veronica P Dubois, Olivia C Sehl, Paula J Foster, John A Ronald
Mol Imaging Biol. 2022 Apr;24(2):298-308. doi: 10.1007/s11307-021-01672-3. Epub 2021 Nov 16.
To compare the cytotoxicity of PFC + and unlabeled CAR-T cells, 5 × 104 NALM6-tdT-FLuc cells were seeded with PFC + or unlabeled CAR-T cells at increasing effector to target ratios (1:4, 1:2, 1:1). Twenty-four hours later, 1 µL of D-luciferin was added to each well (30 mg/ml, Syd Labs), and BLI was performed immediately on an IVIS Lumina XRMS scanner (IVIS Lumina XRMS In Vivo Imaging System, PerkinElmer). BLI signal was evaluated with region-of-interest (ROI) analysis using Living Image Software (PerkinElmer). Quantification was performed by drawing ROIs over each well to obtain the average radiance per well (photons/second/mm2/steradian).

品牌名称:Syd Labs
产品名称:D-荧光素钾盐(D-Luciferin Potassium Salt)
货号:MB000102-R70170或MB102-R70170
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D-荧光素钾盐 Syd Labs MB000102-R70170、D-luciferin Syd Labs、Goldbio D-荧光素钾盐LUCK-1G、Goldbio D-荧光素钾盐LUCK-10G、PE D-荧光素钾盐122799、PE D-荧光素钾盐122799-10、Biosynth D-荧光素钾盐L-8220、Sigma D-荧光素钾盐50227、Promega D-荧光素钾盐E1601
D-荧光素钾盐(D-Luciferin Potassium Salt,CAS号:115144-35-9)相关资讯:
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