SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein (IHU-VM1ST)
(悉得）Syd Labs SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein (IHU-VM1ST) (catalog No. IHU-VM1ST) is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
产品参数
| 货号 | IHU-VM1ST |
|---|---|
| 产品名称 | SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein (IHU-VM1ST) |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | SARS-COV-2 |
| 来源 | HEK293 |
| Accession | QHD43416.1 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions(F817P, A892P, A899P, A942P, K986P, V987P)and alanine substitutions(R683A and R685A)are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. |
| 分子量 | The protein has a predicted MW of 133.60 kDa. Due to glycosylation, the protein migrates to 140-160 kDa based on Tris-Bis PAGE result. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | Shipped at ambient temperature. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Products will be shipped from the warehouse in Massachusetts. Promotion is running from time to time. Welcome to send a request for quote to message@sydlabs.com. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
IHU-VM1ST: SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein (IHU-VM1ST)
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Please remember our product information: SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein (IHU-VM1ST) catalog number: IHU-VM1ST (悉得）Syd Labs