Recombinant Heparinase II Enzyme
Syd Labs Recombinant Heparinase II Enzyme BP000032-GD6, Heparinase II cleaves at the 1-4 linkages between hexosamines and uronic acid residues (both glucuronic and iduronic) of heparin and heparan sulfate (relative activity about 1:2), yielding oligoaccharides (mainly disaccharides). Specific activity of recombinant Heparinase II >6 IU/mg.
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| 货号 | BP001020-Z65-4 |
|---|---|
| 产品名称 | Recombinant Heparinase II Enzyme |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 别称 | Heparin Lyase II; Heparin Eliminase II |
| 来源 | E. coli-derived |
| 形式 | In hepes buffer with 30% glycerol and 0.01 M Ca2+. |
| 纯度 | >95% by SDS-PAGE. |
| 运输 | Recombinant heparinase II is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 注意事项 | Conditions of optimal Heparinase II Enzyme performance should be determined experimentally by the investigator. Custom service to make mixture of Heparinases (I, II, and/or III) is available. |
| 线下下单 | 电话: 18162660842微信: duozhaozhao_2017Email: message@sydlabs.comOr 联系我们 |
描述
BP000032-GD6: Recombinant Heparinase II Enzyme
Background
Heparinase II enzyme cleaves at the 1-4 linkages between hexosamines and uronic acid residues (both glucuronic and iduronic) of heparin and heparan sulfate (relative activity about 1:2), yielding oligoaccharides (mainly disaccharides).
Located in the extracellular matrix, the glycosaminoglycan of heparin plays important physiological roles in anticoagulation and angiogenesis. The acidic polysaccharide of Heparin consists of a heterogeneous disaccharide repeating unit of hexosamine and uronic acid (L-iduronic or D-glucuronic acid) connected through 1-4 linkages and modified with various functional groups.
The three heparinase enzymes (heparinase I, heparinase II, and heparinase III) produced by Flavobacterium heparinum specifically recognize and cleave at different sequences of heparin and heparan sulfate. If interested, please see more information about heparinases and their heparin-related applications.
BP000032-GD6: Recombinant Heparinase II from Flavobacterium heparinum
Source: E. coli-derived
Specific activity >6 IU/mg. One international unit (IU) of recombinant Heparinase II is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparin or heparan sulfate per minute at 30°C and pH 7.3.
Purity >95% by SDS-PAGE.
Molecular weight: 84,100.
Isoelectric point: 8.9 – 9.1.
Form: In hepes buffer with 30% glycerol and 0.01 M Ca2+.
Shipping: Recombinant heparinase II is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
Please remember our product information: Recombinant Heparinase II Enzyme: BP000032-GD6 Syd Labs