Mouse RNF43 Protein C-hFc Tag | Syd Labs BP004521
(悉得）Syd Labs 货号：BP004521 Mouse RNF43 Protein with the C-hFc Tag (小鼠 RNF43 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly24-Tyr197.
产品参数
| 货号 | BP004521 |
|---|---|
| 产品名称 | Mouse RNF43 Protein C-hFc Tag | Syd Labs BP004521 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | E3 ubiquitin-protein ligase RNF43；RING finger protein 43；RING-type E3 ubiquitin transferase RNF43；Rnf43 |
| 物种 | Mouse |
| 来源 | The recombinant Mouse RNF43 Protein, (amino acids Gly24-Tyr197) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q5NCP0 |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Gly24-Tyr197 |
| 分子量 | The recombinant Mouse RNF43 protein with the hFc tag at the C-terminus has a predicted MW of 45.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Mouse R spondin 1 protein with the C-hFc tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Mouse RNF43 protein with the C-hFc tag with the EC50 of 0.11 ug/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Mouse RNF43 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Mouse RNF43 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Mouse RNF43 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004521: Mouse RNF43 Protein with the C-hFc Tag (小鼠 RNF43 蛋白 C-hFc 标签)
RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
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