Mouse IL-2 R alpha/CD25 Protein C-His Tag | Syd Labs BP003734
(悉得）Syd Labs 货号：BP003734 Mouse IL-2 R alpha/CD25 Protein with the C-His Tag (小鼠 IL-2 R α/CD25 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Lys236.
产品参数
| 货号 | BP003734 |
|---|---|
| 产品名称 | Mouse IL-2 R alpha/CD25 Protein C-His Tag | Syd Labs BP003734 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Mouse |
| 来源 | The recombinant Mouse IL-2 R alpha/CD25 Protein, (amino acids Glu22-Lys236) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Glu22-Lys236 |
| 分子量 | The recombinant Mouse IL-2 R alpha/CD25 protein with the His tag at the C-terminus has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Mouse IL-2 R alpha protein with the C-His tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Mouse IL-2 protein with the C-His tag with the EC50 of 0.21 ug/ml determined by ELISA (QC Test). The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Mouse IL-2 R alpha/CD25 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Mouse IL-2 R alpha/CD25 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Mouse IL-2 R alpha/CD25 Protein performance should be determined experimentally by the investigator. |
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文献
BP003734: Mouse IL-2 R alpha/CD25 Protein with the C-His Tag (小鼠 IL-2 R α/CD25 蛋白 C-His 标签)
IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
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