In vivo Grade Recombinant Anti-mouse CD25 Mouse Fab Fragment
Recombinant anti-mouse CD25 mouse IgG2c monoclonal antibodies (clone: PC61, PC61.5 or PC61.5.3) produced from CHO or engineered CHO cell lines good for in vitro and in vivo studies. The afucosylated antibody may behave better in in vivo regulatory T cell depletion.
产品参数
| 货号 | PA007156.mf |
|---|---|
| 产品名称 | In vivo Grade Recombinant Anti-mouse CD25 Mouse Fab Fragment |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 克隆号 | PC61, PC61.5 or PC61.5.3. |
| 免疫源 | IL-2-dependent cytolytic mouse T-cell clone B6.1. |
| 抗体形式 | 0.2 μM filtered solution of 1x PBS. |
| 内毒素 | Less than 1 EU/mg of protein as determined by LAL method. |
| 纯度 | >95% by SDS-PAGE under reducing conditions. |
| 运输 | The in vivo grade recombinant anti-mouse CD25 mouse IgG2c kappa monoclonal antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied. |
| 注意事项 | Recombinant anti-mouse CD25 mouse IgG2c monoclonal antibodies (clone: PC61, PC61.5 or PC61.5.3) produced from CHO or engineered CHO cell lines good for in vitro and in vivo studies. The afucosylated antibody may behave better in in vivo regulatory T cell depletion. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
Background
The in vivo grade recombinant anti-mouse CD25 monoclonal antibodies are based on the rat monoclonal antibody anti-CD25 (mouse) with the clone number of PC61, PC61.5 or PC61.5.3. The rat IgG1 lamda monoclonal antibody has been validated and reported for use in immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. In vivo depletion of mouse CD25(+)CD4(+)FoxP3(+) Treg cells using the rat PC61 monoclonal antibody is widely used to characterize the Treg function. The PC61 monoclonal antibody specifically binds to an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by the 3C7 and 7D4 monoclonal antibodies. By doing so, the antibody blocks binding of IL-2 to CD25.
PA007156.m2c: In Vivo Grade Recombinant Anti-mouse CD25 Mouse IgG2c Kappa Monoclonal Antibody
The in vivo grade recombinant anti-mouse CD25 mouse IgG2c kappa monoclonal antibody was produced in CHO cells.
Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
Syd Labs also provides the following research grade antibody biosimilar proteins:
Daclizumab Biosimilar, research grade, Anti-human CD25 Monoclonal Antibody