Human VAP-1 Protein N-His Tag | Syd Labs BP003526
(悉得)Syd Labs 货号:BP003526 Human VAP-1 Protein with the N-His Tag (人 VAP-1 蛋白 N-His 标签) is expressed from HEK293 with His tag at the N-Terminus.It contains Gly27-Asn763.
产品参数
| 货号 | BP003526 |
|---|---|
| 产品名称 | Human VAP-1 Protein N-His Tag | Syd Labs BP003526 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Human |
| 来源 | The recombinant Human VAP-1 Protein, (amino acids Gly27-Asn763) fused with a His tag at the N-terminus, was produced in HEK 293 cells. |
| Accession | Q16853-1 |
| 产品标签 | N-His |
| 生物素化 | no |
| 确定序列 | Gly27-Asn763 |
| 分子量 | The recombinant Human VAP-1 protein with the His tag at the N-terminus has a predicted MW of 82.88 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human VAP-1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | It is recommended to reconstitute the lyophilized recombinant Human VAP-1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human VAP-1 Protein performance should be determined experimentally by the investigator. |
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文献
BP003526: Human VAP-1 Protein with the N-His Tag (人 VAP-1 蛋白 N-His 标签)
The semicarbazide-sensitive amine oxidase (SSAO), also known as vascular adhesion protein-1 (VAP-1) or primary amine oxidase (PrAO), is a deaminating enzyme highly expressed in vessels that generates harmful products as a result of its enzymatic activity. As a multifunctional enzyme, it is also involved in inflammation through its ability to bind and promote the transmigration of circulating leukocytes into inflamed tissues.
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