Human TSLP Protein C-His-Avi Tag | Syd Labs BP005430
(悉得）Syd Labs 货号：BP005430 Human TSLP Protein with the C-His-Avi Tag (人 TSLP 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159.
产品参数
| 货号 | BP005430 |
|---|---|
| 产品名称 | Human TSLP Protein C-His-Avi Tag | Syd Labs BP005430 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | thymic stromal lymphopoietin; TSLP |
| 物种 | Human |
| 来源 | The recombinant Human TSLP Protein, (amino acids Tyr29-Gln159) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q969D9-1 |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Tyr29-Gln159 |
| 分子量 | The recombinant Human TSLP protein with the His-Avi tag at the C-terminus has a predicted MW of 17.8 kDa. Due to furin cleavage site, the protein migrates to 12 kDa&20-25 kDa based on Bis-Tris PAGE result. |
| 活性 | Immobilized recombinant Human TSLP protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TSLPR protein with the C-hFc tag with the EC50 of 50.8 ng/ml determined by ELISA (QC Test). The affinity constant of 9.21 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human TSLP Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human TSLP Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human TSLP Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005430: Human TSLP Protein with the C-His-Avi Tag (人 TSLP 蛋白 C-His-Avi 标签)
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
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