Human Transferrin R/CD71 Protein N-His-Avi Tag | Syd Labs BP005670

Human Transferrin R/CD71 Protein BP005670 Syd Labs

Human Transferrin R/CD71 Protein N-His-Avi Tag | Syd Labs BP005670

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(悉得)Syd Labs 货号:BP005670 Human Transferrin R/CD71 Protein with the N-His-Avi Tag (人转铁蛋白 R/CD71 蛋白 N-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.

SKU: BP005670 分类: ,

产品参数

货号 BP005670
产品名称Human Transferrin R/CD71 Protein N-His-Avi Tag | Syd Labs BP005670
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
物种 Human
来源 The recombinant Human Transferrin R/CD71 Protein, (amino acids Cys89-Phe760) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Accession P02786
产品标签 N-His-Avi
生物素化 no
确定序列 Cys89-Phe760
分子量 The recombinant Human Transferrin R/CD71 protein with the His-Avi tag at the N-terminus has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-80 kDa based on Bis-Tris PAGE result.
活性 Immobilized recombinant Human Transferrin R protein with the N-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Transferrin R Antiboy protein with the N-hFc tag with the EC50 of 9.2 ng/ml determined by ELISA (QC Test). The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human Transferrin R/CD71 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human Transferrin R/CD71 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human Transferrin R/CD71 Protein performance should be determined experimentally by the investigator.
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文献

BP005670: Human Transferrin R/CD71 Protein with the N-His-Avi Tag (人转铁蛋白 R/CD71 蛋白 N-His-Avi 标签)

The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
请记住我们的产品信息: 人转铁蛋白 R/CD71 蛋白 (N-His-Avi 标签)BP005670 Syd Labs(Human Transferrin R/CD71 Protein with the N-His-Avi Tag).