Human TNFSF15 Trimer Protein N-His-Flag Tag | Syd Labs BP005610
Syd Labs 货号：BP005610 Human TNFSF15 Trimer Protein with the N-His-Flag Tag （人 TNFSF15 三聚体蛋白 N-His-Flag 标签）
产品参数
| 货号 | BP005610 |
|---|---|
| 产品名称 | Human TNFSF15 Trimer Protein N-His-Flag Tag | Syd Labs BP005610 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A |
| 物种 | Human |
| 来源 | The recombinant Human TNFSF15 Trimer Protein, (amino acids Asp91-Leu251) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells. |
| 产品标签 | N-His-Flag |
| 确定序列 | Asp91-Leu251 |
| 分子量 | The recombinant Human TNFSF15 Trimer protein with the His-Flag tag at the N-terminus has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human TNFSF15 Trimer protein with the N-His-Flag tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-TNFSF15 Antibody protein with the N-hFc tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test). The affinity constant of 3.06 nM as determined in SPR assay (Biacore T200). |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human TNFSF15 Trimer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human TNFSF15 Trimer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human TNFSF15 Trimer Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005610: Human TNFSF15 Trimer Protein with the N-His-Flag Tag (人 TNFSF15 三聚体蛋白 N-His-Flag 标签)
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
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