Human TNFSF12/TWEAK Protein C-hFc Tag | Syd Labs BP003809
Syd Labs 货号：BP003809 Human TNFSF12/TWEAK Protein with the C-hFc Tag （人 TNFSF12/TWEAK 蛋白 C-hFc 标签）
产品参数
| 货号 | BP003809 |
|---|---|
| 产品名称 | Human TNFSF12/TWEAK Protein C-hFc Tag | Syd Labs BP003809 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Human |
| 来源 | The recombinant Human TNFSF12/TWEAK Protein, (amino acids Ser43-His249) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-hFc |
| 确定序列 | Ser43-His249 |
| 分子量 | The recombinant Human TNFSF12/TWEAK protein with the hFc tag at the C-terminus has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
| 活性 | Immobilized recombinant Human TNFSF12 protein with the C-hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-TNFSF12 Antibody protein with the C-hFc tag with the EC50 of 0.14 ug/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human TNFSF12/TWEAK Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human TNFSF12/TWEAK Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human TNFSF12/TWEAK Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP003809: Human TNFSF12/TWEAK Protein with the C-hFc Tag(人 TNFSF12/TWEAK 蛋白 C-hFc 标签)
Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation.
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