Human Siglec-10 Protein C-His Tag | Syd Labs BP005413
(悉得）Syd Labs 货号：BP005413 Human Siglec-10 Protein with the C-His Tag (人 Siglec-10 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Met17-Thr546.
产品参数
| 货号 | BP005413 |
|---|---|
| 产品名称 | Human Siglec-10 Protein C-His Tag | Syd Labs BP005413 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | SLG2; SIGLEC10; MGC126774; PRO940 |
| 物种 | Human |
| 来源 | The recombinant Human Siglec-10 Protein, (amino acids Met17-Thr546) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q96LC7-1 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Met17-Thr546 |
| 分子量 | The recombinant Human Siglec-10 protein with the His tag at the C-terminus has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human Siglec-10 protein with the C-His tag at 1 ug/ml (100 ul/Well). Dose response curve for the recombinant Anti-Siglec-10 Antibody protein with the C-hFc tag with the EC50 of 2.3 ng/ml determined by ELISA (QC Test). The affinity constant of 15.9 uM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in 20mM PB, 150mM NaCl, 25mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Siglec-10 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human Siglec-10 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human Siglec-10 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005413: Human Siglec-10 Protein with the C-His Tag (人 Siglec-10 蛋白 C-His 标签)
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
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