Human MICB Protein C-His-Avi Tag | Syd Labs BP005513
(悉得）Syd Labs 货号：BP005513 Human MICB Protein with the C-His-Avi Tag (人 MICB 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala23-Gly298.
产品参数
| 货号 | BP005513 |
|---|---|
| 产品名称 | Human MICB Protein C-His-Avi Tag | Syd Labs BP005513 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | MICB; MIC-B; PERB11.2 |
| 物种 | Human |
| 来源 | The recombinant Human MICB Protein, (amino acids Ala23-Gly298) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q29980-1 |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Ala23-Gly298 |
| 分子量 | The recombinant Human MICB protein with the His-Avi tag at the C-terminus has a predicted MW of 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human MICB protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-MICB Antibody protein with the C-hFc tag with the EC50 of 42.4 ng/ml determined by ELISA. The affinity constant of 83.31 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human MICB Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human MICB Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human MICB Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005513: Human MICB Protein with the C-His-Avi Tag (人 MICB 蛋白 C-His-Avi 标签)
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
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