Human LIF R/CD118 Protein C-His Tag | Syd Labs BP004555

Human LIF R/CD118 Protein BP004555 Syd Labs

Human LIF R/CD118 Protein C-His Tag | Syd Labs BP004555

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(悉得)Syd Labs 货号:BP004555 Human LIF R/CD118 Protein with the C-His Tag (人 LIF R/CD118 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ser833.

SKU: BP004555 分类: ,

产品参数

货号 BP004555
产品名称Human LIF R/CD118 Protein C-His Tag | Syd Labs BP004555
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 FLJ99923; SJS2; STWS; SWS; LIFR; CD118; FLJ98106
物种 Human
来源 The recombinant Human LIF R/CD118 Protein, (amino acids Gln45-Ser833) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-His
生物素化 no
确定序列 Gln45-Ser833
分子量 The recombinant Human LIF R/CD118 protein with the His tag at the C-terminus has a predicted MW of 90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human LIF protein with the C-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human LIF R protein with the C-His tag with the EC50 of 0.30 ug/ml determined by ELISA (QC Test). The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human LIF R/CD118 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 It is recommended to reconstitute the lyophilized recombinant Human LIF R/CD118 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human LIF R/CD118 Protein performance should be determined experimentally by the investigator.
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文献

BP004555: Human LIF R/CD118 Protein with the C-His Tag (人 LIF R/CD118 蛋白 C-His 标签)

 

The leukemia inhibitory factor receptor (LIF-R) subunit is a component of cell-surface receptor complexes for the multifunctional cytokines, LIF, cardiotrophin-1, ciliary neurotrophic factor, and human oncostatin M. The structure of the human LIF-R gene is similar to that of the mouse gene. The transmembrane receptor is encoded by 19 exons.

请记住我们的产品信息: 人 LIF R/CD118 蛋白 (C-His 标签)BP004555 Syd Labs(Human LIF R/CD118 Protein with the C-His Tag).