Human LAP (TGF beta 1) Protein N-His Tag | Syd Labs BP003555
(悉得）Syd Labs 货号：BP003555 Human LAP (TGF beta 1) Protein with the N-His Tag (人 LAP （TGF beta 1） 蛋白 N-His 标签) is expressed from HEK293 with His tag at the N-Terminus.It contains Leu30-Arg278(C33S).
产品参数
| 货号 | BP003555 |
|---|---|
| 产品名称 | Human LAP (TGF beta 1) Protein N-His Tag | Syd Labs BP003555 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Human |
| 来源 | The recombinant Human LAP (TGF beta 1) Protein, (amino acids Leu30-Arg278(C33S)) fused with a His tag at the N-terminus, was produced in HEK 293 cells. |
| Accession | P01137 |
| 产品标签 | N-His |
| 生物素化 | no |
| 确定序列 | Leu30-Arg278(C33S) |
| 分子量 | The recombinant Human LAP (TGF beta 1) protein with the His tag at the N-terminus has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human LAP (TGF beta 1) protein with the N-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human ITGAV&ITGB6 protein with the N-His tag with the EC50 of 1.0 ug/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human LAP (TGF beta 1) Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human LAP (TGF beta 1) Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human LAP (TGF beta 1) Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP003555: Human LAP (TGF beta 1) Protein with the N-His Tag (人 LAP （TGF beta 1） 蛋白 N-His 标签)
Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex.
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