Human IL-27RA/TCCR Protein C-hFc Tag | Syd Labs BP005260
(悉得)Syd Labs 货号:BP005260 Human IL-27RA/TCCR Protein with the C-hFc Tag (人 IL-27RA/TCCR 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly34-Lys516.
产品参数
| 货号 | BP005260 |
|---|---|
| 产品名称 | Human IL-27RA/TCCR Protein C-hFc Tag | Syd Labs BP005260 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | WSX1; IL27RA; CRL1; TCCR; Cytokine receptor-like 1; IL27R alpha; IL27R; WSX-1 |
| 物种 | Human |
| 来源 | The recombinant Human IL-27RA/TCCR Protein, (amino acids Gly34-Lys516) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Gly34-Lys516 |
| 分子量 | The recombinant Human IL-27RA/TCCR protein with the hFc tag at the C-terminus has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human IL-27 RA protein with the C-hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IL-27 protein with the C-His tag with the EC50 of 55.9 ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human IL-27RA/TCCR Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human IL-27RA/TCCR Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human IL-27RA/TCCR Protein performance should be determined experimentally by the investigator. |
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文献
BP005260: Human IL-27RA/TCCR Protein with the C-hFc Tag (人 IL-27RA/TCCR 蛋白 C-hFc 标签)
A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects.
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