Human IL-25/IL-17E Protein C-His Tag | Syd Labs BP004766
(悉得）Syd Labs 货号：BP004766 Human IL-25/IL-17E Protein with the C-His Tag (人 IL-25/IL-17E 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177.
产品参数
| 货号 | BP004766 |
|---|---|
| 产品名称 | Human IL-25/IL-17E Protein C-His Tag | Syd Labs BP004766 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25 |
| 物种 | Human |
| 来源 | The recombinant Human IL-25/IL-17E Protein, (amino acids Tyr33-Gly177) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Tyr33-Gly177 |
| 分子量 | The recombinant Human IL-25/IL-17E protein with the His tag at the C-terminus has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. |
| 活性 | The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human IL-25/IL-17E Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human IL-25/IL-17E Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human IL-25/IL-17E Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004766: Human IL-25/IL-17E Protein with the C-His Tag (人 IL-25/IL-17E 蛋白 C-His 标签)
IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
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