Human IL-13 Protein N-hFc Tag | Syd Labs BP004836

Human IL-13 Protein BP004836 Syd Labs

Human IL-13 Protein N-hFc Tag | Syd Labs BP004836

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(悉得)Syd Labs 货号:BP004836 Human IL-13 Protein with the N-hFc Tag (人 IL-13 蛋白 N-hFc 标签) is expressed from HEK293 with hFc tag at the N-Terminus.It contains Gly21-Asn132.

SKU: BP004836 分类: ,

产品参数

货号 BP004836
产品名称Human IL-13 Protein N-hFc Tag | Syd Labs BP004836
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
物种 Human
来源 The recombinant Human IL-13 Protein, (amino acids Gly21-Asn132) fused with a hFc tag at the N-terminus, was produced in HEK 293 cells.
Accession AAK53823
产品标签 N-hFc
生物素化 no
确定序列 Gly21-Asn132
分子量 The recombinant Human IL-13 protein with the hFc tag at the N-terminus has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Biotinylated Human IL-13Ra1 protein with the N-hFc tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human IL-13 protein with the N-hFc tag with the EC50 of 15.8 ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human IL-13 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human IL-13 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human IL-13 Protein performance should be determined experimentally by the investigator.
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文献

BP004836: Human IL-13 Protein with the N-hFc Tag (人 IL-13 蛋白 N-hFc 标签)

 

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

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