Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein C-His-Avi Tag | Syd Labs BP005219
(悉得）Syd Labs 货号：BP005219 Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein with the C-His-Avi Tag (人 HLA-E*01：03&B2M&肽 （VMAPRTLVL） 四聚体蛋白 C-His-Avi 标签) is assembled by biotinylated monomer and streptavidin.It contains Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide.
产品参数
| 货号 | BP005219 |
|---|---|
| 产品名称 | Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein C-His-Avi Tag | Syd Labs BP005219 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1 |
| 物种 | Human |
| 来源 | The recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein, (amino acids Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide |
| 分子量 | The recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer protein with the His-Avi tag at the C-terminus has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| 活性 | The affinity constant of 17.61 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005219: Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein with the C-His-Avi Tag (人 HLA-E*01：03&B2M&肽 （VMAPRTLVL） 四聚体蛋白 C-His-Avi 标签)
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
请记住我们的产品信息: 人 HLA-E*01：03&B2M&肽 （VMAPRTLVL） 四聚体蛋白 （C-His-Avi 标签）BP005219 Syd Labs（Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein with the C-His-Avi Tag）.