Human Coagulation Factor III/Tissue Factor Protein C-His Tag | Syd Labs BP003880
(悉得）Syd Labs 货号：BP003880 Human Coagulation Factor III/Tissue Factor Protein with the C-His Tag (人凝血因子 III/组织因子蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Ser33-Glu251.
产品参数
| 货号 | BP003880 |
|---|---|
| 产品名称 | Human Coagulation Factor III/Tissue Factor Protein C-His Tag | Syd Labs BP003880 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Human |
| 来源 | The recombinant Human Coagulation Factor III/Tissue Factor Protein, (amino acids Ser33-Glu251) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | P13726-1 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Ser33-Glu251 |
| 分子量 | The recombinant Human Coagulation Factor III/Tissue Factor protein with the His tag at the C-terminus has a predicted MW of 25.89 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
| 活性 | Immobilized recombinant Human Coagulation Factor III protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Coagulation Factor III Antibody protein with the C-hFc tag with the EC50 of 4.9 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Coagulation Factor III/Tissue Factor Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human Coagulation Factor III/Tissue Factor Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human Coagulation Factor III/Tissue Factor Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP003880: Human Coagulation Factor III/Tissue Factor Protein with the C-His Tag (人凝血因子 III/组织因子蛋白 C-His 标签)
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.
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