Human CD98 Protein N-His Tag | Syd Labs BP005069
(悉得）Syd Labs 货号：BP005069 Human CD98 Protein with the N-His Tag (人 CD98 蛋白 N-His 标签) is expressed from HEK293 with His tag at the N-Terminus.It contains Arg206-Ala630.
产品参数
| 货号 | BP005069 |
|---|---|
| 产品名称 | Human CD98 Protein N-His Tag | Syd Labs BP005069 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 4F2hc; CD98; SLC3A2; 4F2; CD98HC; MDU1; NACAE; 4T2HC |
| 物种 | Human |
| 来源 | The recombinant Human CD98 Protein, (amino acids Arg206-Ala630) fused with a His tag at the N-terminus, was produced in HEK 293 cells. |
| 产品标签 | N-His |
| 生物素化 | no |
| 确定序列 | Arg206-Ala630 |
| 分子量 | The recombinant Human CD98 protein with the His tag at the N-terminus has a predicted MW of 47.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human CD98 protein with the N-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-CD98 Antibody protein with the N-hFc tag with the EC50 of 10.1 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human CD98 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human CD98 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human CD98 Protein performance should be determined experimentally by the investigator. |
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文献
BP005069: Human CD98 Protein with the N-His Tag (人 CD98 蛋白 N-His 标签)
The type II transmembrane protein CD98, best known as the heavy chain of the heterodimeric amino acid transporters (HAT), is required for the surface expression and basolateral localization of this transporter complex in polarized epithelial cells. CD98 also interacts with beta1 integrins resulting in an increase in their affinity for ligand. In this study we explored the role of the transmembrane and cytoplasmic domains of CD98 on integrin-dependent cell adhesion and migration in polarized renal epithelial cells.
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