Human CD117 Protein C-hFc Tag | Syd Labs BP003460
(悉得)Syd Labs 货号:BP003460 Human CD117 Protein with the C-hFc Tag (人 CD117 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln26-Pro520.
产品参数
| 货号 | BP003460 |
|---|---|
| 产品名称 | Human CD117 Protein C-hFc Tag | Syd Labs BP003460 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Human |
| 来源 | The recombinant Human CD117 Protein, (amino acids Gln26-Pro520) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | P10721-2 |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Gln26-Pro520 |
| 分子量 | The recombinant Human CD117 protein with the hFc tag at the C-terminus has a predicted MW of 82.46 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human SCF protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human CD117 protein with the C-hFc tag with the EC50 of 12.4 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human CD117 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human CD117 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human CD117 Protein performance should be determined experimentally by the investigator. |
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文献
BP003460: Human CD117 Protein with the C-hFc Tag (人 CD117 蛋白 C-hFc 标签)
The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.
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