Human Alkaline Phosphatase (Germ type) /ALPG Protein C-mFc Tag | Syd Labs BP003374

Human Alkaline Phosphatase (Germ type) /ALPG Protein BP003374 Syd Labs

Human Alkaline Phosphatase (Germ type) /ALPG Protein C-mFc Tag | Syd Labs BP003374

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(悉得)Syd Labs 货号:BP003374 Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-mFc Tag (人碱性磷酸酶(胚芽型)/ALPG 蛋白 C-mFc 标签) is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ile20-Asp503.

SKU: BP003374 分类: ,

产品参数

货号 BP003374
产品名称Human Alkaline Phosphatase (Germ type) /ALPG Protein C-mFc Tag | Syd Labs BP003374
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
物种 Human
来源 The recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein, (amino acids Ile20-Asp503) fused with a mFc tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-mFc
生物素化 no
确定序列 Ile20-Asp503
分子量 The recombinant Human Alkaline Phosphatase (Germ type) /ALPG protein with the mFc tag at the C-terminus has a predicted MW of 78.24 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human Alkaline Phosphatase (Germ type)rotein with the C-mFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Alkaline Phosphatase Antibody protein with the C-hFc tag with the EC50 of 29.3 ng/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein performance should be determined experimentally by the investigator.
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文献

BP003374: Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-mFc Tag (人碱性磷酸酶(胚芽型)/ALPG 蛋白 C-mFc 标签)

 

Alkaline phosphatase can be considered “our favorite enzyme” for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

请记住我们的产品信息: 人碱性磷酸酶(胚芽型)/ALPG 蛋白 (C-mFc 标签)BP003374 Syd Labs(Human Alkaline Phosphatase (Germ type) /ALPG Protein with the C-mFc Tag).