Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM4FOB)
(悉得）Syd Labs Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM4FOB) (catalog No. COV-VM4FOB) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
产品参数
| 货号 | COV-VM4FOB |
|---|---|
| 产品名称 | Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM4FOB) |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | S protein; Spike glycoprotein; S glycoprotein; COVID-19 |
| 物种 | SARS-COV-2 |
| 来源 | HEK293 |
| Accession | QHO60594.1 |
| 产品标签 | C-His-Avi |
| 生物素化 | yes |
| 确定序列 | Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F) |
| 分子量 | The protein has a predicted MW of 136.70 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Tris-Bis PAGE result. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | Shipped at ambient temperature. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Products will be shipped from the warehouse in Massachusetts. Promotion is running from time to time. Welcome to send a request for quote to message@sydlabs.com. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
COV-VM4FOB: Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM4FOB)
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Please remember our product information: Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM4FOB) catalog number: COV-VM4FOB (悉得）Syd Labs