Biotinylated Human FGFR2 beta (IIIc) Protein C-His-Avi Tag | Syd Labs BP004509
Syd Labs 货号:BP004509 Biotinylated Human FGFR2 beta (IIIc) Protein with the C-His-Avi Tag (生物素化人 FGFR2 β (IIIc) 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg152-Glu377.
产品参数
| 货号 | BP004509 |
|---|---|
| 产品名称 | Biotinylated Human FGFR2 beta (IIIc) Protein C-His-Avi Tag | Syd Labs BP004509 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM |
| 物种 | Human |
| 来源 | The recombinant Biotinylated Human FGFR2 beta (IIIc) Protein, (amino acids Arg152-Glu377) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | yes |
| 确定序列 | Arg152-Glu377 |
| 分子量 | The recombinant Biotinylated Human FGFR2 beta (IIIc) protein with the His-Avi tag at the C-terminus has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human FGF basic protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human FGFR2 beta (IIIc) protein with the C-His tag with the EC50 of 1.25 ug/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Biotinylated Human FGFR2 beta (IIIc) Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Biotinylated Human FGFR2 beta (IIIc) Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Biotinylated Human FGFR2 beta (IIIc) Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品,不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题,欢迎随时联系Syd Labs国内市场推广合作伙伴:武汉多找找科技有限公司,企业微信:duozhaozhao2024 联系电话:18162581039(龙经理) |
文献
BP004509: Biotinylated Human FGFR2 beta (IIIc) Protein with the C-His-Avi Tag (生物素化人 FGFR2 β (IIIc) 蛋白 C-His-Avi 标签)
Four distinct genes encoding closely related FGF receptors, FGF R1 – 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 – 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.
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