Biotinylated Human EGFR/HER1 Protein C-His-Avi Tag | Syd Labs BP005627
(悉得)Syd Labs 货号:BP005627 Biotinylated Human EGFR/HER1 Protein with the C-His-Avi Tag (生物素化人 EGFR/HER1 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser645.
产品参数
| 货号 | BP005627 |
|---|---|
| 产品名称 | Biotinylated Human EGFR/HER1 Protein C-His-Avi Tag | Syd Labs BP005627 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
| 物种 | Human |
| 来源 | The recombinant Biotinylated Human EGFR/HER1 Protein, (amino acids Leu25-Ser645) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | yes |
| 确定序列 | Leu25-Ser645 |
| 分子量 | The recombinant Biotinylated Human EGFR/HER1 protein with the His-Avi tag at the C-terminus has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Biotinylated Human EGFR protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate(5 ug/ml). Dose response curve for the recombinant Human EGF protein with the C-hFc tag with the EC50 of 5.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.62 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Biotinylated Human EGFR/HER1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Biotinylated Human EGFR/HER1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Biotinylated Human EGFR/HER1 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品,不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题,欢迎随时联系Syd Labs国内市场推广合作伙伴:武汉多找找科技有限公司,企业微信:duozhaozhao2024 联系电话:18162581039(龙经理) |
文献
BP005627: Biotinylated Human EGFR/HER1 Protein with the C-His-Avi Tag (生物素化人 EGFR/HER1 蛋白 C-His-Avi 标签)
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
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