Biotinylated Human EGFR/HER1 Protein C-His-Avi Tag | Syd Labs BP005627

Biotinylated Human EGFR/HER1 Protein BP005627 Syd Labs

Biotinylated Human EGFR/HER1 Protein C-His-Avi Tag | Syd Labs BP005627

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(悉得)Syd Labs 货号:BP005627 Biotinylated Human EGFR/HER1 Protein with the C-His-Avi Tag (生物素化人 EGFR/HER1 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser645.

SKU: BP005627 分类: ,

产品参数

货号 BP005627
产品名称Biotinylated Human EGFR/HER1 Protein C-His-Avi Tag | Syd Labs BP005627
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
物种 Human
来源 The recombinant Biotinylated Human EGFR/HER1 Protein, (amino acids Leu25-Ser645) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-His-Avi
生物素化 yes
确定序列 Leu25-Ser645
分子量 The recombinant Biotinylated Human EGFR/HER1 protein with the His-Avi tag at the C-terminus has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Biotinylated Human EGFR protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate(5 ug/ml). Dose response curve for the recombinant Human EGF protein with the C-hFc tag with the EC50 of 5.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.62 nM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Biotinylated Human EGFR/HER1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Biotinylated Human EGFR/HER1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Biotinylated Human EGFR/HER1 Protein performance should be determined experimentally by the investigator.
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文献

BP005627: Biotinylated Human EGFR/HER1 Protein with the C-His-Avi Tag (生物素化人 EGFR/HER1 蛋白 C-His-Avi 标签)

 

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

请记住我们的产品信息: 生物素化人 EGFR/HER1 蛋白 (C-His-Avi 标签)BP005627 Syd Labs(Biotinylated Human EGFR/HER1 Protein with the C-His-Avi Tag).