Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein C-hFc Tag | Syd Labs BP005292
Syd Labs 货号：BP005292 Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein with the C-hFc Tag is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22 – Asp117(CD3E)&Phe22-Ala105(CD3D).
产品参数
| 货号 | BP005292 |
|---|---|
| 产品名称 | Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein C-hFc Tag | Syd Labs BP005292 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
| 物种 | Cynomolgus |
| 来源 | The recombinant Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein, (amino acids Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D)) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-hFc |
| 生物素化 | yes |
| 确定序列 | Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D) |
| 分子量 | The recombinant Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta protein with the hFc tag at the C-terminus has a predicted MW of 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa and 44-46 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Anti-CD3 protein with the C-hFc tag Antibody at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Cynomolgus CD3E&CD3D protein with the C-hFc tag with the EC50 of 58.2 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005376: Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein with the C-hFc Tag
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
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