Biotinylated Cynomolgus CD3E/CD3 epsilon Protein C-His Tag | Syd Labs BP003454

Biotinylated Cynomolgus CD3E/CD3 epsilon Protein BP003454 Syd Labs

Biotinylated Cynomolgus CD3E/CD3 epsilon Protein C-His Tag | Syd Labs BP003454

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Syd Labs 货号:BP003454 Biotinylated Cynomolgus CD3E/CD3 epsilon Protein with the C-His Tag

SKU: BP003454 分类: ,

产品参数

货号 BP003454
产品名称Biotinylated Cynomolgus CD3E/CD3 epsilon Protein C-His Tag | Syd Labs BP003454
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
物种 Cynomolgus
来源 The recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon Protein, (amino acids Gln22-Asp117) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-His
生物素化 yes
确定序列 Gln22-Asp117
分子量 The recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon protein with the His tag at the C-terminus has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 13-16 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Anti-CD3 Antibody protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Cynomolgus CD3E protein with the C-His tag with the EC50 of 0.87 ng/ml determined by ELISA.
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon Protein performance should be determined experimentally by the investigator.
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文献

BP003454: Biotinylated Cynomolgus CD3E/CD3 epsilon Protein with the C-His Tag

 
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

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