重组抗小鼠CD19单抗(1D3)流式 | Syd Labs PA007364.r2a
Syd Labs重组抗小鼠CD19单克隆抗体(克隆号1D3) ，大鼠IgG2a Kappa(货号：PA007364.r2a)是用哺乳动物细胞生产的重组抗体，可用于流式细胞术和免疫组织化学-冷冻等研究，纯度>95%。其不变区为大鼠Rat IgG2a Kappa (rIgG2a或r2a)，可与重组大鼠IgG2a同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007364.r2a |
|---|---|
| 产品名称 | 重组抗小鼠CD19单抗(1D3)流式 | Syd Labs PA007364.r2a |
| 英文名 | Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3), Rat IgG2a Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | B 淋巴细胞抗原 CD19、分化簇 19、B 淋巴细胞表面抗原 B4、T 细胞表面抗原 Leu-12、CVID3 |
| 概述 | Syd Labs提供重组大鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | 1D3 |
| 同种型 | 大鼠 IgG2a kappa |
| 特异性 | 该重组 1D3 抗体与小鼠 CD19 蛋白特异性结合 |
| 免疫源 | 重组抗小鼠CD19单克隆抗体 (克隆：1D3)在哺乳动物细胞中产生 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，含0.09%叠氮化钠 |
| 偶联 | 非偶联 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 重组抗小鼠CD19单克隆抗体(克隆号1D3) ，大鼠IgG2a Kappa用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 12个月，保存在2 至 8°C。 请勿冻结。 |
| 注意事项 | PA007364.r2a Syd Labs提供重组大鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | 流式细胞术（FC）和免疫组织化学-冷冻（IHC-F）。 |
文献
重组抗小鼠CD19单克隆抗体(克隆号1D3) ，大鼠IgG2a Kappa（Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3), Rat IgG2a Kappa，货号：PA007364.r2a Syd Labs）
1D3抗体可与小鼠CD19蛋白结合。CD19是一种跨膜蛋白，表达于包括小鼠浆细胞在内的所有B谱系细胞表面。CD19在小鼠B细胞中发挥两大主要作用：一是作为衔接蛋白将胞浆信号蛋白招募至细胞膜，二是协同CD19/CD21复合体降低B细胞受体（BCR）信号通路的激活阈值。CD19是B淋巴细胞发育及淋巴瘤诊断的重要生物标志物，同时也可作为白血病免疫治疗的靶点。
抗小鼠CD19单抗(1D3)流式部分参考文献:
1. Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus
Muller N, et al. Mol Ther Methods Clin Dev. 2022 Jul 8;26:131-141. PMID: 35755944
“Cells were stained with FITC-conjugated anti-CD19 mAb (clone 1D3) and analyzed by flow cytometry to monitor B cell depletion. The depletion efficiency was >95% in peripheral blood as determined by flow cytometry using 1D3 antibody. Peripheral blood was collected and stained with anti-CD19 (1D3) and anti-CD3 antibodies…”
2. Therapeutic efficacy of anti-CD19 CAR-T cells in a mouse model of systemic lupus erythematosus
Ma J, et al. Cell Mol Immunol. 2021 Aug;18(8):1896-1903. PMID: 32472023
“For flow cytometry, cells were stained with anti-CD19 (clone 1D3) antibody to identify B cells. The percentage of CD19+ B cells was significantly reduced in treated mice. Spleen cells were isolated and stained with 1D3 antibody for CD19 expression…”
3. Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells
Kochenderfer JF, et al. Blood. 2010 Nov 11;116(19):3875-86. PMID: 20631379
“B cells were identified by staining with PE-conjugated anti-CD19 (clone 1D3) antibody. Flow cytometry analysis showed eradication of CD19+ cells in lymphoma. The antibody 1D3 was used to gate B cell population in bone marrow…”
4. Self-renewal of B-1 lymphocytes is dependent on CD19
Krop I, et al. Eur J Immunol. 1996 Jan;26(1):238-42. PMID: 8566073
“Cells were stained with anti-CD19 mAb (1D3) for flow sorting. Peritoneal cells were analyzed for CD19 expression using 1D3. The antibody 1D3 was used in all flow cytometry experiments for B cell identification…”
5. The signaling activity of murine CD19 is regulated during cell development
Krop I, et al. J Immunol. 1996 Jul 1;157(1):48-56. PMID: 8683154
“Murine B cells were stained with biotinylated 1D3 anti-CD19 mAb followed by streptavidin-PE. Flow cytometry was used to assess CD19 expression levels during development. The 1D3 clone was specific for murine CD19…”