D-荧光素钾盐(D-Luciferin Potassium Salt)引用文献合集与应用指南
在小鼠活体成像(In Vivo Imaging System, IVIS)和体外生物发光检测中,D-荧光素钾盐(D-Luciferin Potassium Salt)作为荧光素酶(Luciferase)催化底物,凭借其高灵敏度和低背景噪音特性,已成为肿瘤转移追踪、CAR-T免疫治疗效果评估及基因表达监测不可或缺的生物化学试剂。
美国 Syd Labs 生产的高纯度D-荧光素钾盐(货号:MB102 或 MB000102-R70170,CAS号:115144-35-9,纯度>99% HPLC),多年来被全球众多顶尖高校与科研机构的核心实验室(Core Facilities)采购与持续复购。截至目前,已有 107 篇高水平SCI学术文献(发表于 Nature Communications, Cancer Discovery, eLife, Advanced Science 等顶级期刊)引用并证实了其卓越的生物发光性能与质量稳定性(文献库持续更新中):
Syd Labs D-荧光素钾盐权威引用文献合集(全107篇)
- Aging of mice is associated with p16 (Ink4a)-and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cells. Brandon M Hall, et al. Aging (Albany NY). 2016. PMCID: PMC4993332
- Murine mesenchymal cells that express elevated levels of the CDK inhibitor p16(Ink4a) in vivo are not necessarily senescent. David Frescas, et al. Cell Cycle. 2017. PMCID: PMC5584871
- p16 (Ink4a) and senescence-associated β-galactosidase can be induced in macrophages as part of a reversible response to physiological stimuli. Brandon M Hall, et al. Aging (Albany NY). 2017. PMCID: PMC5611982
- Induction of a tier-1-like phenotype in diverse tier-2 isolates by agents that guide HIV-1 Env to perturbation-sensitive, nonnative states. Jacklyn Johnson, et al. J Virol. 2017. PMCID: PMC5512248
- Accurate predictions of population-level changes in sequence and structural properties of HIV-1 Env using a volatility-controlled diffusion model. Orlando DeLeon, et al. PLoS Biol. 2017. PMCID: PMC5383018
- Interleukin-17 promotes metastasis in an immunocompetent orthotopic mouse model of prostate cancer. David Cunningham, et al. Am J Clin Exp Urol. 2018. PMCID: PMC6055074
- Multimodality cellular and molecular imaging of concomitant tumour enhancement in a syngeneic mouse model of breast cancer metastasis. Katie M Parkins, et al. Sci Rep. 2018. PMCID: PMC5997674
- Nontoxic targeting of energy metabolism in preclinical VM-M3 experimental glioblastoma. Zachary M Augur, et al. Front Nutr. 2018. PMCID: PMC6186985
- Inhibition of yes‑associated protein suppresses brain metastasis of human lung adenocarcinoma in a murine model. Ping‑Chih Hsu, et al. J Cell Mol Med. 2018. PMCID: PMC5980132
- Cellular MRI reveals altered brain arrest of genetically engineered metastatic breast cancer cells. Katie M Parkins, et al. Contrast Media Mol Imaging. 2019. PMCID: PMC6348811
- Cucurbitacin E inhibits the Yes‑associated protein signaling pathway and suppresses brain metastasis of human non‑small cell lung cancer in a murine model. Ping-Chih Hsu, et al. Oncol Rep. 2019. PMCID: PMC6610039
- Shortened ex vivo manufacturing time of EGFRvIII-specific chimeric antigen receptor (CAR) T cells reduces immune exhaustion and enhances antiglioma therapeutic function. Hillary G Caruso, et al. J Neurooncol. 2019. PMID: 31686330
- The pro-tumor effect of CD200 expression is not mimicked by agonistic CD200R antibodies. Zofia Pilch, et al. PLoS One. 2019. PMCID: PMC6336379
- Circulating tumor cells exhibit metastatic tropism and reveal brain metastasis drivers. Remi Klotz, et al. Cancer Discov. 2019. PMCID: PMC6954305
- Properties of HIV-1 env and human seminal fluid that determine virus inhibition by antibodies and microbicides. Johnson, Jacklyn, et al. Summer 2019.
- Th17 cells promote tumor growth in an immunocompetent orthotopic mouse model of prostate cancer. Zhenling Duan, et al. Am J Clin Exp Urol. 2019. PMCID: PMC6734037
- Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila. Ane Martin Anduaga, et al. eLife. 2019. PMCID: PMC6890466
- Cucurbitacin E inhibits the Yes-associated protein signaling pathway and suppresses brain metastasis of human non-small cell lung cancer in a murine model. Ping-Chih Hsu, et al. Oncol Rep. 2019. PMCID: PMC6610039
- LINE1 derepression in aged wild type and SIRT6 deficient mice drives inflammation. Matthew Simon, et al. Cell Metab. 2019. PMCID: PMC6449196
- Engineering Circulating Tumor Cells as Novel Cancer Theranostics. Katie M Parkins, et al. Theranostics. 2020. PMCID: PMC7359075
- Local targeting of lung-tumor-associated macrophages with pulmonary delivery of a CSF-1R inhibitor for the treatment of breast cancer lung metastases. Sulaiman S Alhudaithi, et al. Mol Pharm. 2020. PMCID: PMC8060921
- The fusion peptide proximal region of the HIV-1 envelope glycoproteins regulates exposure of immunogenic epitopes at the trimer base. Hillel Haim, et al. bioRxiv. 2020.
- Engineering “self-homing” circulating tumour cells as novel cancer theranostics. Katie M Parkins, et al. Theranostics. 2020. PMCID: PMC7359075
- Tumor-responsive, multifunctional CAR-NK cells cooperate with impaired autophagy to infiltrate and target glioblastoma. Jiao Wang, et al. bioRxiv. 2020.
- Intra-arterial injection to create bone metastasis of prostate cancer in mice. Lin Zhong, et al. Am J Clin Exp Urol. 2020. PMCID: PMC7486538
- Local Targeting of Lung Tumor Associated Macrophages with Pulmonary Delivery of a CSF-1R Inhibitor for the Treatment of Breast Cancer Lung Metastases. Sulaiman S Alhudaithi, et al. Mol Pharm. 2020. PMCID: PMC8060921
- Phosphatidylserine receptors enhance SARS-CoV-2 infection. Dana Bohan, et al. PLoS Pathog. 2021. PMCID: PMC8641883
- Visualizing CAR-T cell Immunotherapy Using 3 Tesla Fluorine-19 MRI. Veronica P Dubois, et al. Mol Imaging Biol. 2021. PMCID: PMC8983548
- Targeting CA-125 Transcription by Development of a Conditionally Replicative Adenovirus for Ovarian Cancer Treatment. Er Yue, et al. Cancers (Basel). 2021. PMCID: PMC8428227
- Pulmonary administration of a CSF-1R inhibitor alters the balance of tumor-associated macrophages and supports first-line chemotherapy in a lung cancer model. Hanming Zhang, et al. Int J Pharm. 2021. PMID: 33545279
- Inhibition of aberrant tissue remodelling by mesenchymal stromal cells singly coated with soft gels presenting defined chemomechanical cues. Sing Wan Wong, et al. Nat Biomed Eng. 2021. PMCID: PMC8908879
- Site-specific antibody-drug conjugates with variable drug-to-antibody-ratios for AML therapy. Zhefu Dai, et al. J Control Release. 2021. PMCID: PMC8373670
- PRDX-1 supports the survival and antitumor activity of primary and CAR-modified NK cells under oxidative stress. Marta Klopotowska, et al. Cancer Immunol Res. 2021. PMCID: PMC9414282
- Modular cell-assembled adipose matrix-derived bead foams as a mesenchymal stromal cell delivery platform for soft tissue regeneration. Pascal Morissette Martin, et al. Biomaterials. 2021. PMID: 34182328
- Site-Specific Antibody-Drug Conjugates with Variable Drug-to-Antibody-Ratios for Acute Myeloid Leukemia Therapy. Zhefu Dai, et al. J Control Release. 2021. PMCID: PMC8373670
- Effect of Cholesterol Content of Lipid Composition in mRNA-LNPs on the Protein Expression in the Injected Site and Liver After Local Administration in Mice. Maho Kawaguchi, et al. J Pharm Sci. 2022. PMCID: PMC9805379
- Stability Study of mRNA-Lipid Nanoparticles Exposed to Various Conditions Based on the Evaluation between Physicochemical Properties and Their Relation with Protein Expression Ability. Mariko Kamiya, et al. Pharmaceutics. 2022. PMCID: PMC9697436
- Engineered natural killer cells impede the immunometabolic CD73-adenosine axis in solid tumors. Andrea M Chambers, et al. eLife. 2022. PMCID: PMC9342955
- Anti-tumor antibody isotype response can be modified with locally administered immunoadjuvants. Adam A Walters, et al. Drug Deliv Transl Res. 2022. PMCID: PMC10238356
- Cancer immunotherapy based on image-guided STING activation by nucleotide nanocomplex-decorated ultrasound microbubbles. Xuefeng Li, et al. Nat Nanotechnol. 2022. PMCID: PMC9378430
- Development of highly effective anti-mesothelin hYP218 chimeric antigen receptor T cells with increased tumor infiltration and persistence for treating solid tumors. Sakshi Tomar, et al. Mol Cancer Ther. 2022. PMCID: PMC9256778
- Dormant senescence-prone cells (DSPC): a lifetime memory about systemic genotoxic stress. Andrei Gudkov, et al. Research Square. 2022.
- Commonly elicited antibodies against the base of the HIV-1 Env trimer guide the population-level evolution of a structure-regulating region in gp41. Hillel Haim, et al. J Virol. 2022. PMCID: PMC9278142
- A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis. Lucía Zhu, et al. EMBO Mol Med. 2022. PMCID: PMC8899920
- Limited variation between SARS-CoV-2-infected individuals in domain specificity and relative potency of the antibody response against the spike glycoprotein. Hanora A Van Ert, et al. Microbiol Spectr. 2022. PMCID: PMC8791189
- A drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent local relapse and treat established brain metastasis. Lucía Zhu, et al. EMBO Mol Med. 2022. PMCID: PMC8899920
- Cryopreservation of Plasmodium Sporozoites. Carson Bowers, et al. Pathogens. 2022. PMCID: PMC9784981
- A clinically compatible drug‐screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis. Lucía Zhu, et al. EMBO Mol Med. 2022. PMCID: PMC8899920
- Increased Activity of a NK-Specific CAR-NK Framework Targeting CD3 and CD5 for T-Cell Leukemias. Elisaveta Voynova, et al. Cancers (Basel). 2022. PMCID: PMC8833462
- Anti-Trypanosoma cruzi activity of Coptis rhizome extract and its constituents. Yuki Tayama, et al. Trop Med Health. 2023. PMCID: PMC9976467
- Induction of liver-resident memory T cells and protection at liver-stage malaria by mRNA-containing lipid nanoparticles. Sayuri Nakamae, et al. Front Immunol. 2023. PMCID: PMC10482405
- Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids. Junchen Liu, et al. Nat Commun. 2023. PMCID: PMC9884228
- A Case Study of Chimeric Antigen Receptor T Cell Function: Donor Therapeutic Differences in Activity and Modulation with Verteporfin. Jiyong Liang, et al. Cancers (Basel). 2023. PMCID: PMC9953964
- Evaluation of a DoE based approach for comprehensive modelling of the effect of lipid nanoparticle composition on nucleic acid delivery. Yue Qin, et al. Biomaterials. 2023. PMID: 37243988
- Influence of lipid composition of messenger RNA-loaded lipid nanoparticles on the protein expression via intratracheal administration in mice. Longjian Geng, et al. Int J Pharm. 2023. PMID: 36972778
- UVB modifies skin immune-stroma cross-talk and promotes effector T cell recruitment during cryptic Leishmania donovani infection. Paul M Kaye, et al. bioRxiv. 2023.
- An Image-Based Identification of Aggressive Breast Cancer Circulating Tumor Cell Subtypes. Mohamed Kamal, et al. Cancers (Basel). 2023. PMCID: PMC10216536
- Visualizing cell–cell communication using synthetic notch activated MRI. TianDuo Wang, et al. Proc Natl Acad Sci U S A. 2023. PMCID: PMC10089199
- Delivery of human adipose-derived stromal cells within in situ forming chitosan/PEG-PTMC hydrogels induces adverse outcomes in a femoral artery ligation model in athymic nu/nu mice. Lauren E. Flynn, et al. bioRxiv. 2023.
- Machine learning identifies experimental brain metastasis subtypes based on their influence on neural circuits. Alberto Sanchez-Aguilera, et al. Cancer Cell. 2023. PMCID: PMC10507426
- Protocol to generate murine organotypic brain cultures for drug screening and evaluation of anti-metastatic efficacy. Lucía Zhu, et al. STAR Protoc. 2023. PMCID: PMC10120397
- Adaptive rewiring of purine metabolism promotes treatment resistance in H3K27M-mutant diffuse midline glioma. Wahl DR, et al. Res Sq [Preprint]. 2023. PMCID: PMC10543500
- synNotch-programmed iPSC-derived NK cells usurp TIGIT and CD73 activities for glioblastoma therapy. Kyle B Lupo, et al. Nat Commun. 2024. PMCID: PMC10907695
- Biohybrid microrobots locally and actively deliver drug-loaded nanoparticles to inhibit the progression of lung metastasis. Fangyu Zhang, et al. Sci Adv. 2024. PMCID: PMC11168470
- Macrophage-derived developmental endothelial locus 1 (DEL-1) expression promotes an immunoprotective phenotype in experimental visceral leishmaniasis. Jakub Lukaszonek, et al. bioRxiv. 2024.
- Radiation Synergizes with IL2/IL15 Stimulation to Enhance Innate Immune Activation and Antitumor Immunity. Xuefeng Li, et al. Mol Cancer Ther. 2024. PMID: 37956421
- GTP signaling links metabolism, DNA repair, and responses to genotoxic stress. Weihua Zhou, et al. Cancer Discov. 2024. PMCID: PMC10872631
- Humanized anti-CD11d monoclonal antibodies suitable for basic research and therapeutic applications. Eoin N Blythe, et al. Antib Ther. 2024. PMCID: PMC11744312
- Phosphoproteomic Profiling Reveals mTOR Signaling in Sustaining Macrophage Phagocytosis of Cancer Cells. Bixin Wang, et al. Cancers (Basel). 2024. PMCID: PMC11674635
- Amino Acid and Glucose Fermentation Maintain ATP Content in Mouse and Human Malignant Glioma Cells. Derek C Lee, et al. ASN Neuro. 2024. PMCID: PMC11792161
- Prostate Specific Membrane Antigen Expression in a Syngeneic Breast Cancer Mouse Model. Aditi A Shirke, et al. Mol Imaging Biol. 2024. PMCID: PMC11281974
- Purine salvage promotes treatment resistance in H3K27M-mutant diffuse midline glioma. Wahl DR, et al. Cancer Metab. 2024. PMCID: PMC11003124
- Adipose-Derived Stromal Cells Delivered in Chitosan/PEG-PTMC Hydrogels Induce Adverse Outcomes in Nude Mice with Femoral Artery Ligation. Amsden BG, et al. GEN Biotechnology. 2024.
- Inhibition of H3K27M-enhanced ATM signaling increases radiation efficacy in diffuse midline glioma. Parsels JD, et al. bioRxiv. 2024.
- Sequence-based prediction of virus phenotype, evolutionary potential, and selective pressures. Chávez RAR, et al. The University of Iowa. 2024.
- CD47 Knock‑Out Using CRISPR‑Cas9 RNA Lipid Nanocarriers Results in Reduced Mesenchymal Glioblastoma Growth In Vivo. Nadia Rouatbi, et al. Adv Sci (Weinh). 2025. PMCID: PMC11948039
- The impact of a high fat diet and platelet activation on pre-metastatic niche formation. Marta Hergueta-Redondo, et al. Nat Commun. 2025. PMCID: PMC11965330
- Probing the effects of polysaccharide hydrogel composition on the viability and pro-angiogenic function of human adipose-derived stromal cells. Fiona E Serack, et al. J Biomed Mater Res A. 2025. PMID: 39304971
- CAR macrophages with built-In CD47 blocker combat tumor antigen heterogeneity and activate T cells via cross-presentation. Siqi Chen, et al. Nat Commun. 2025. PMCID: PMC12043996
- The SRG rat as a novel host for an orthotopic patient-derived xenograft model of breast cancer brain metastasis. Niveen Fulcher, et al. Sci Rep. 2025. PMCID: PMC12216210
- Characterization of Bone Marrow-Derived Immune Compartment in Murine Brain Metastases by Spectral Flow Cytometry. Ailen D Garcia-Santillan, et al. Methods Mol Biol. 2025. PMCID: PMC12281579
- STAT3 sustains tumorigenicity following mutant KRAS ablation. D’Amico S, et al. EMBO Rep. 2025. PMCID: PMC12549880
- A Site-Specific MiniAp4-Trastuzumab Conjugate Prevents Brain Metastasis. Masmudi-Martín M, et al. Mol Pharm. 2025. PMCID: PMC11881140
- Genetically reprogrammed exosomes for immunotherapy of acute myeloid leukemia. Zhang L, et al. Molecular Therapy. 2025.
- Surfaceome CRISPR activation screening uncovers ligands regulating tumor sensitivity to NK cell killing. Cong L, et al. Nat Commun. 2026. PMCID: PMC13538616
- Tumor cell dissemination is facilitated through regulatory T cell-driven extracellular matrix remodeling. Garcia-Santillan AD, et al. bioRxiv. 2025. PMCID: PMC12633443
- Enzymatic Cellular Nanoparticles Deliver Payloads across Mucosal Barriers. Zhang L, et al. Bioconjug Chem. 2025. PMID: 40675932
- Focused Ultrasound-mediated Disruption of Plasma Protein Binding Enhances Chemotherapeutic Effects of Paclitaxel on Xenografted Ovarian Cancer in Mice. SS Yoo, et al. Anticancer Research. 2025.
- Combined quetiapine and radiation therapy approach to treat mesothelioma-initiating cells and increase survival in a mouse model of mesothelioma. Cardenas A, et al. bioRxiv. 2025.
- A versatile platform for chemical engineering of exosomes empowered by ADP-ribosyl cyclases. Zhang L, et al. Nature Communications. 2025.
- Bi-directional highways and super-seeder tissues underpin parasite dissemination in experimental Leishmania donovani infection. Paul M Kaye, et al. Nature Communications. 2026.
- Investigating Trypanosoma cruzi infection and pathology following suppressive drug treatment. S Jayawardhana, et al.
- Lactate cannot replace glucose for maintaining the viability of mouse and human glioma cells. Ta NL, et al. bioRxiv. 2025.
- Innate Immune-Cloaked Microgel-Coated Mesenchymal Stromal Cells Reverse Persistent Pulmonary Fibrosis via Reparative Macrophages. Cho IS, et al. Adv Mater. 2026. PMID: 40984773
- PolyA tail segmentation improves the stability of the template DNA and increases the translatability of in vitro transcribed mRNA. Spiewla T, et al. Nucleic Acids Res. 2026. PMCID: PMC12805897
- Imaging CRISPR-edited CAR-T cell therapies with optical and positron emission tomography reporters. Sanchez-Pupo RE, et al. Theranostics. 2026. PMCID: PMC12846014
- ITGB1 Regulates Triple-Negative Breast Cancer Development by Modulating the Tumor Microenvironment. Song N, et al. Advanced Science. 2026.
- Quiescent tumor cells shape the immunosuppressive microenvironment in pancreatic ductal adenocarcinoma. Matsui W, et al. Research Square.
- Activation of Thrombin Activatable Fibrinolysis Inhibitor by Thrombomodulin Attenuates Metastasis of Breast Cancer Cells. Reza T, et al. The University of Western Ontario. 2026.
- Implanted Dynamic Electric Field Therapy for Glioblastoma: Preclinical Safety and Efficacy of Intratumoral Modulation Therapy. Akbarzadeh AH, et al. Neuro-Oncology Advances. 2026.
- Ketogenic diet as a metabolic vehicle enhancing the therapeutic efficacy of mebendazole and devimistat in juvenile syngeneic high-grade glioma. Stopka SA, et al. Cell Reports Medicine. 2026.
- Comprehensive Mapping of the Virus and Host Factors that Guide the Paths of HIV-1 Escape from a Therapeutic. Haim H, et al. Cell Rep. 2026. PMID: 42322610
- Quiescent tumor cells shape the immunosuppressive microenvironment in pancreatic cancer. Matsui W, et al. Nat Commun. 2026. PMID: 42481500
- Human Decellularized Adipose Tissue Hydrogels as a Delivery Platform to Enhance Human Endothelial Colony-Forming Cell Retention and Vascular Regeneration. Terek AE, Walker JT, Bell GI, et al. Journal of Functional Biomaterials. 2026.
- FcBP-HFQ lipid-mediated trastuzumab modification of mRNA-loaded lipid nanoparticles enhances delivery to HER2-expressing cancer cells. Matsumoto M, Takayama R, Matsuguchi C, et al. European Journal of Pharmaceutical Sciences. 2026.
- USP15 regulates mitotic fidelity, metastatic potential, and chemotherapeutic response in ovarian cancer cells. Ogunsanya A, Alfaran F, Amadu ON, Padmanabhan A. Mol Ther Oncol. 2026. PMCID: PMC13571140
- Clinically Relevant Transplantable Tumor Models for Studying Metastatic Ovarian Cancer and Therapeutic Response in Mouse. Padmanabhan A. J Vis Exp. 2026. PMID: 42258493

D-荧光素钾盐的发光原理与实验配置指南
1. 生物发光机制
D-荧光素钾盐(D-Luciferin Potassium Salt)在三磷酸腺苷(ATP)和Mg²⁺存在条件下,被荧光素酶(Luciferase)催化氧化,生成激发态的氧代荧光素(Oxyluciferin),并在释放波长约 560 nm(体外)至 600 nm(体内,由于组织吸收和散射红移)的黄绿色荧光。由于哺乳动物内源性无生物发光背景,该系统具备极高的信噪比。
2. 小鼠活体成像配置与使用浓度
- 储存母液配置:使用无菌无钙镁 PBS(pH 7.4)将 D-荧光素钾盐溶解配制成 15 mg/mL(约 47.1 mM)的母液。过滤除菌后,避光分装于 -20°C 或 -80°C 保存,避免反复冻融。
- 小鼠注射剂量:按小鼠体重计算,标准腹腔注射(i.p.)剂量为 150 mg/kg(即每 20 g 体重小鼠注射 200 μL 的 15 mg/mL 溶液)。
- 成像峰值窗口:腹腔注射后 10–20 分钟内荧光信号达到平台期,建议在此时间窗口内进行光学成像检测。
主流 D-荧光素钾盐 进口品牌选型参考
科研人员在进行实验室试剂采购时,可根据纯度、质量稳定度与性价比对比以下主流品牌:
- Syd Labs D-荧光素钾盐 (MB102 / MB000102-R70170):高纯度(>99%),广泛应用于欧美科研核心平台,具备极高性价比与数百篇高分文献验证。
- Goldbio D-荧光素钾盐 (LUCK-100):生命科学领域常用活体成像底物品牌。
- PerkinElmer (PE) D-荧光素钾盐 (122799-100MG):小鼠成像仪(如 IVIS Spectrum)配套品牌。
- Promega D-荧光素钾盐 (E1601-5mg):体外荧光素酶报告基因检测的经典品牌。
- Biosynth D-荧光素钾盐 (L-8220) 与 Sigma D-荧光素钾盐 (50227-1mg):老牌生化试剂供应商。
技术支持与授权代理联系方式
如果您对 Syd Labs D-荧光素钾盐的实验配置、活体成像应用有任何技术疑问,或需要申请 Syd Labs 中国代理商 授权资质、确认代理真实性及进行服务反馈,欢迎联系多找找团队:
- 企业微信:duozhaozhao2024
- 联系电话:18162581039(龙经理)