重组抗小鼠CD8b单抗(YTS 156.7) | Syd Labs PA007382.r2b
体内实验级重组抗小鼠CD8b单抗(克隆号:YTS 156.7,货号:PA007382.r2b),大鼠IgG2b Kappa 是用哺乳动物细胞生产的重组抗体,从大鼠抗小鼠CD8b单克隆抗体(克隆号:YTS 156.7)可变区序列中提取,可用于蛋白质印迹、免疫组织化学 (IHC)、流式细胞术 (FC) 和体内CD8+T细胞耗竭(清除)等研究。Syd Labs PA007382.r2b抗小鼠CD8b抗体不变区为大鼠IgG2b kappa (rIgG2b或r2b),可与重组大鼠IgG2b同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007382.r2b |
|---|---|
| 产品名称 | 重组抗小鼠CD8b单抗(YTS 156.7) | Syd Labs PA007382.r2b |
| 英文名 | In vivo Grade Recombinant Anti-mouse CD8b Monoclonal Antibody, Rat IgG2b Kappa (Clone: YTS 156.7) |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD8β,T细胞表面糖蛋白CD8β链,CD_抗原CD8b |
| 概述 | 重组抗小鼠CD8b单克隆抗体是用哺乳动物细胞生产的重组抗体,从大鼠抗小鼠CD8b单克隆抗体(克隆号:YTS 156.7)可变区序列中提取的,适合体外和体内研究。 |
| 克隆号 | YTS 156.7 |
| 同种型 | 大鼠IgG2b, kappa |
| 特异性 | CD8b |
| 抗体形式 | 0.2 μM过滤溶液,1x PBS |
| 内毒素 | 根据 LAL 方法,≤1 EU每1mg 蛋白质 |
| 纯度 | >95%(在还原条件下通过SDS-PAGE测定) |
| 运输 | 体内实验级重组抗小鼠CD8b单克隆抗体,大鼠IgG2b Kappa(克隆号YTS 156.7)用冰袋运输。收到后,请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 如果保存在2 至 8°C,自收到之日起可保存1个月。 如果保存在-20 至 -70°C,自收到之日起可保存 12个月。 |
| 注意事项 | PA007382.r2b Syd Labs重组抗小鼠CD8b单克隆抗体是用哺乳动物细胞生产的重组抗体,从大鼠抗小鼠CD8b单克隆抗体(克隆号:YTS 156.7)可变区序列中提取的,适合体外和体内研究。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品,不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题,欢迎随时联系Syd Labs国内市场推广合作伙伴:武汉多找找科技有限公司,企业微信:duozhaozhao2024 联系电话:18162581039(龙经理) |
| 应用详情 | 蛋白质印迹、免疫组织化学(IHC)、流式细胞术(FC)和体内CD8+T细胞耗竭(清除)。 |
文献
PA007382.r2b: Syd Labs体内实验级重组抗小鼠CD8b单克隆抗体(克隆号YTS 156.7),大鼠IgG2b Kappa(In vivo Grade Recombinant Anti-mouse CD8b Monoclonal Antibody, Rat IgG2b Kappa (Clone: YTS 156.7)))
大鼠抗小鼠 CD8b 单克隆抗体 YTS 156.7(大鼠 IgG2b kappa)可与小鼠 CD8b 蛋白(T 细胞表面糖蛋白 CD8 beta 链、Lyt-3 或 Ly-3)发生反应,该蛋白由小鼠 *cd8b* 基因编码,负责编码二聚体 CD8 蛋白的 CD8b 链。小鼠 CD8 蛋白主要负责细胞介导的免疫防御和 T 细胞发育。
我们的重组 YTS 156.7 抗体包含大鼠抗小鼠 CD8b 单克隆抗体(杂交瘤克隆名称或编号:YTS 156.7)的部分(可变区)或完整氨基酸序列。
抗小鼠 CD8b 单克隆抗体(克隆号:YTS 156.7)参考文献:
1. Structure, function, and immunomodulation of the CD8 co-receptor.
Srinivasan, et al. Front Immunol. 2024 Sep 3;15:1412513. PMID: 39253084; PMCID: PMC11381289
“Expressed on the surface of CD8+ T cells, the CD8 co-receptor is a key component that contributes to antigen recognition, immune cell maturation, and immune cell signaling. … This review outlines the unique structure and function of different CD8 domains in the context of the distinct properties of CD8?? homodimers and CD8?? heterodimers. … Additionally, the applications of anti-CD8 monoclonal antibodies targeting CD8 are summarized to highlight translational research for targeted immunotherapy.”
2. Genetic and commensal induction of IL-18 drive intestinal epithelial MHCII via IFN?.
Van Der Kraak, et al. Mucosal Immunol. 2021 Sep;14(5):1100-1112. PMID: 34103660; PMCID: PMC8562907
“Major Histocompatibility Complex Class II (MHCII) is dynamically expressed on intestinal epithelial cells throughout the intestine, but its regulation remains poorly understood. … We observed that spontaneous upregulation of epithelial MHCII correlated with serum Interleukin (IL)-18 and was driven by an intestinal IL-18-IFN? axis. … This process activated or expanded type 1 lymphocytes, suggesting a crucial link between intestinal dysbiosis, systemic inflammation, and epithelial antigen presentation.”
3. Multiplex Screening Assay for Identifying Cytotoxic CD8+ T Cell Epitopes.
Poh, et al. Front Immunol. 2020 Mar 17;11:400. PMID: 32218786; PMCID: PMC7078160
“The cytotoxicity of epitope-specific CD8+ T cells is usually measured indirectly through IFN? production, but direct measurement assays have historically been limited in multiplexing capacity. … Here, we develop a multiplex cytotoxicity assay that allows direct, simultaneous measurement of up to 23 different specificities of CD8+ T cells in a single reaction. … This technique is highly useful for studying both human and mouse CD8+ T cells, providing new possibilities for comprehensive analyses of CD8+ T cell cytotoxicity in vaccine evaluations.”

