重组抗小鼠CD4单抗(GK1.5)流式 | Syd Labs PA007494.r2b
Syd Labs重组抗小鼠CD4单克隆抗体(克隆号GK1.5) ，大鼠IgG2b Kappa(货号：PA007494.r2b)是用哺乳动物细胞生产的重组抗体，可用于流式细胞术和免疫组织化学-冷冻等研究，纯度>95%。其不变区为大鼠Rat IgG2b Kappa (rIgG2b或r2b)，可与重组大鼠IgG2b同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007494.r2b |
|---|---|
| 产品名称 | 重组抗小鼠CD4单抗(GK1.5)流式 | Syd Labs PA007494.r2b |
| 英文名 | Recombinant Anti-mouse CD4 Monoclonal Antibody (Clone: GK1.5), Rat IgG2b Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | L3T4, Ly-4, Leu-3, T4 |
| 概述 | Syd Labs提供重组大鼠IgG2b同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | GK1.5 |
| 同种型 | 大鼠 IgG2b kappa |
| 特异性 | 重组 GK1.5 抗体与小鼠 T 细胞受体 CD4 特异性结合 |
| 免疫源 | 重组抗小鼠CD4单克隆抗体 (克隆：GK1.5)在哺乳动物细胞中产生 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，含0.09%叠氮化钠 |
| 偶联 | 非偶联 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 重组抗小鼠CD4单克隆抗体(克隆号GK1.5) ，大鼠IgG2b Kappa用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 12个月，保存在2 至 8°C。 请勿冻结。 |
| 注意事项 | PA007494.r2b Syd Labs提供重组大鼠IgG2b同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | 流式细胞术（FC）和免疫组织化学-冷冻（IHC-F）。 |
文献
重组抗小鼠CD4单克隆抗体(克隆号GK1.5) ，大鼠IgG2b Kappa（Recombinant Anti-mouse CD4 Monoclonal Antibody (Clone: GK1.5), Rat IgG2b Kappa，货号：PA007494.r2b Syd Labs）
抗小鼠CD4单抗（GK1.5抗体）是一种特异性靶向小鼠CD4分子的经典大鼠单克隆抗体，其天然宿主同型为Rat IgG2b Kappa。CD4主要表达于辅助性T细胞、调节性T细胞（Tregs）以及部分树突状细胞表面，作为主要的共受体参与MHC II类分子介导的抗原识别和T细胞活化信号转导。在免疫学基础研究及临床前动物模型中，抗小鼠CD4单抗因其出色的细胞耗竭与阻断功能，被广泛用于探索辅助性T细胞在抗肿瘤免疫、自身免疫性疾病、抗感染免疫以及移植耐受中的核心调控机制。
作为优质的生命科学试剂供应商，Syd Labs推出的重组抗小鼠CD4单抗（GK1.5抗体）采用高效的哺乳动物细胞表达系统生产，具备极高的纯度与批间一致性。该产品专为流式细胞术以及要求严苛的体内实验级研究设计，能够特异性识别小鼠CD4+细胞并介导高效的体内清除效应，且批次内质量稳定、内毒素水平极低，为您在复杂的动物活体及免疫表型分析实验中提供可靠的科研工具。
抗小鼠CD4单抗(GK1.5)流式部分参考文献:
1. ImmunoPET Imaging of Murine CD4+ T Cells Using Anti-CD4 Cys-Diabody: Effects of Protein Dose on T Cell Function and Imaging
Freise, A. C., et al. Mol Imaging Biol. 2017 Aug;19(4):599-609. PMID: 27966069
“GK1.5 cys-diabody (cDb), a previously developed anti-mouse CD4 antibody fragment, was tested at different doses to assess its effects on positron emission tomography (PET) imaging and CD4+ T cell viability, proliferation, CD4 expression, and function. In vivo administration of GK1.5 cDb at the high dose of 40 µg caused a transient decrease in CD4 expression in spleen, blood, lymph nodes, and thymus, which recovered within 3 days post-injection; this effect was reduced, although not abrogated, when 2 µg was administered…”
2. Migrant memory B cells secrete luminal antibody in the vagina
Oh, J.E., et al. Nature. 2019 Jul;571(7763):122-126. PMID: 31189952
“Cells were surface stained with anti-CD3ε (145–2C11), anti-CD4 antibodies (GK1.5 and RM4–5), and anti-CD44 (IM7), and then fixed and permeabilized using a Cytofix/Cytoperm™ Fixation/Permeabilization kit according to the manufacturer’s instructions. To deplete CD4 T cells, mice were treated with 300 µg intravenously on day −4, −1 and 10 µg anti-mouse CD4 Ab (GK1.5) intravaginally for four consecutive days before virus challenge…”
3. Effect of a CD4-depleting antibody on the development of Cryptococcus neoformans-induced allergic bronchopulmonary mycosis in mice
Arora, S., et al. Infect Immun. 2006 Jul;74(7):4339-48. PMID: 16790808
“Mice were treated with 300 μg of anti-CD4 monoclonal antibody (MAb) GK1.5 intraperitoneally on the day prior to C. neoformans inoculation and boosted with 100 μg of MAb at days 7 and 14. The efficiency of T-cell depletion was assessed by flow cytometric analysis using anti-CD4 Ab RM 4-4, which binds to a region of CD4 distinct from GK1.5…”
4. Protocol for the isolation of mouse senescence-associated CD4+ T cells using flow cytometry and functional assays
Fukushima, Y., et al. STAR Protoc. 2023 Sep 15;4(3):102472. PMID: 37515759
“CD4 MicroBeads do not interfere with anti-CD4 Ab (clone GK1.5) in the staining panels (Tables 1 and 2) and thus are compatible. Anti-CD4 (GK1.5), FITC (1:200)” is listed in the Key resources table under Antibodies. Anti-CD4 (GK1.5), APC-Cy7 (1:200)” is listed in the Key resources table under Antibodies…”
5. T4+ T helper cell function in vivo: differential requirement for induction of antiviral cytotoxic T-cell and antibody responses
Ahmed, R., et al. J Virol. 1988 Jun;62(6):2102-2106. PMID: 2966865
“Two monoclonal antibodies (GKI.5 and RL172.4) directed against the L3T4 (T4) molecule were used for depleting T helper cells from mice. The rat anti-mouse hybridoma MAb GK1.5 was obtained from the American Type Culture Collection, Rockville, Md. For in vivo treatment with GK1.5, mice were injected intraperitoneally (i.p.) with a total of 1.5 mg of GK1.5 antibody…”