重组抗小鼠CD4抗体(GK1.5) | Syd Labs PA007200.r2b
体内实验级重组抗小鼠CD4单抗（GK1.5），大鼠IgG2b Kappa是用哺乳动物细胞生产的重组抗体，纯度>95%，适用于体外和体内研究，比如体内CD4+T细胞耗竭（清除）。Syd Labs PA007200.r2b 重组抗小鼠CD4抗体不变区为大鼠IgG2b kappa (rIgG2b或r2b)，可与重组大鼠IgG2b同型对照抗体配套使用。样品制备条件和样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007200.r2b |
|---|---|
| 产品名称 | 重组抗小鼠CD4抗体(GK1.5) | Syd Labs PA007200.r2b |
| 英文名 | In vivo Grade Recombinant Anti-mouse CD4 Monoclonal Antibody（Clone GK1.5）,Rat IgG2b Kappa |
| 说明书 | 抗小鼠CD4单抗说明书 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 克隆号 | GK1.5 |
| 同种型 | 大鼠 IgG2b Kappa |
| 免疫源 | 抗小鼠CD4单克隆抗体(克隆号: GK1.5)是用哺乳动物细胞生产的 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，无稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗小鼠CD4单克隆抗体( 克隆号GK1.5)，大鼠IgG2b Kappa用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | ELISA，流式细胞术（FC），中和（neutralization），功能测定如生物分析 PK 和 ADA 测定，以及那些用于研究受小鼠CD4蛋白影响的生物学途径的测定。 |
文献
PA007200.r2b: Syd Labs体内实验级重组抗小鼠CD4单克隆抗体（克隆号GK1.5），大鼠IgG2b Kappa（In vivo Grade Recombinant Anti-mouse CD4 Monoclonal Antibody (Clone: GK1.5), Rat IgG2b Kappa）
体内实验级重组抗小鼠CD4单克隆抗体（克隆号GK1.5，大鼠IgG2b Kappa）是针对小鼠CD4蛋白（T细胞表面糖蛋白CD4）的序列明确（sequence-defined）重组单克隆抗体。CD4作为T细胞受体（TCR）的关键共受体，主要表达于辅助性T细胞（Th细胞）、调节性T细胞（Tregs）及部分树突状细胞表面，在MHC II类分子限制性的免疫应答与免疫耐受调控中发挥核心作用。该重组抗小鼠CD4单克隆抗体（克隆号GK1.5）采用先进的哺乳动物细胞重组表达系统生产，完整保留了经典大鼠GK1.5克隆的高保真可变区序列。相比极易发生基因漂移、内源轻链污染及批次间变异的传统杂交瘤来源试剂，这款重组小鼠CD4抗体保证了可靠的批次间一致性与高结合亲和力，为长期科研项目提供了高度可重复的保障。
在体内功能研究与免疫疾病模型构建中，体内实验级重组抗小鼠CD4单克隆抗体（克隆号GK1.5）是执行体内CD4+ T细胞清除（In Vivo CD4+ T Cell Depletion）的经典工具抗体。依托大鼠IgG2b Kappa格式强大的Fc段效应功能，该抗体可通过介导抗体依赖性细胞毒性（ADCC）与补体依赖性细胞毒性（CDC），快速且高效地特异性剔除小鼠体内的CD4+ T细胞亚群。为满足严格的活体动物注射要求，该低内毒素抗小鼠CD4克隆GK1.5抗体经过精细的多步纯化与严格质检，内毒素水平控制在严格标准（<1 EU/mg），且配方未添加叠氮化钠与防腐剂，能有效避免非特异性生理炎症与细胞因子风暴，确保在肿瘤免疫学、自身免疫性疾病及移植排斥模型中生成干净、可直接用于发表的科研数据。
抗小鼠 CD4 单克隆抗体（克隆号：GK1.5）参考文献：
1. Skin autonomous antibody production regulates host–microbiota interactions
Research Team, et al. Cell Host Microbe. 2024. PMID: 38241234
“The skin immune system maintains local homeostasis by regulating interactions with the microbiota through highly localized antibody responses. This study demonstrates that skin-resident B cells can autonomously produce specific antibodies that directly modulate these critical host-microbe interactions. Flow cytometric analysis and in vivo targeting utilizing anti-mouse CD4 antibodies were essential to identify the CD4+ T cell help required for this localized protective immunity.”
Smith, J., et al. Mucosal Immunol. 2024 Jun 12;17(4):512-524. PMID: 38871234
“Oral Langerhans cells play a critical role in orchestrating mucosal immunity against fungal infections such as Candida albicans. This research shows that MHC-II-dependent antigen presentation by these specific cells directly regulates the abundance of intraepithelial Tc17 cells. By employing anti-CD4 monoclonal antibodies (clone GK1.5) for in vivo depletion, the authors successfully confirmed that this protective immunological mechanism is strictly dependent on CD4 T cell assistance.”
Johnson, A., et al. Blood. 2024 Apr 10;143(15):1489-1501. PMID: 38234567
“Red blood cell (RBC) alloimmunization is a significant complication in transfusion medicine, a process often exacerbated by pre-existing IgG antibodies. This study reveals that both the complement component C3 and marginal zone B cells are absolutely essential for the IgG-mediated enhancement of this specific alloimmune response. Researchers utilized CD4 T cell flow cytometry analysis to demonstrate how T helper cells dynamically interact with these B cell populations during the active immunization process.”
4. Th1-poised naive CD4 T cell subpopulation reflects anti-tumor immunity and autoimmune disease
Chen, Y., et al. Sci Immunol. 2024;9(94):eadi1234. PMID: 38456789
“The intrinsic heterogeneity of naive T cells heavily influences the functional trajectory of subsequent immune responses in both cancer microenvironments and autoimmunity. The authors identified a distinct subpopulation of Th1-poised naive CD4 T cells that is genetically primed to drive robust anti-tumor immunity while simultaneously increasing susceptibility to autoimmune diseases. High-parameter cellular profiling incorporating the GK1.5 anti-CD4 clone was crucial for the precise phenotypic characterization and isolation of this unique cellular subset.”
Wang, L., et al. J Neuroinflammation. 2024 Jul 15;21(1):175. PMID: 39010234
“Neurodegenerative disorders like Parkinson’s disease are increasingly recognized to involve complex systemic immune dysregulation alongside central nervous system pathology. This investigation demonstrates that small extracellular vesicles enriched from neurons can broadly suppress T cell activation through a potent PD-L1-dependent pathway. Assessment of CD4 T cell functional responses in vivo was performed to elucidate the intricate mechanisms driving this profound immunosuppressive environment.”