重组抗人CD4单抗(SK3 / Anti-Leu 3a) | Syd Labs PA007386.m1
体内实验级重组抗人CD4单克隆抗体，小鼠IgG1 Kappa（克隆号：SK3 / Anti-Leu 3a，货号：PA007386.m1），纯度>95%，适用于体外和体内研究。Syd Labs PA007386.m1不变区为小鼠Mouse IgG1 Kappa (mIgG1或m1)。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007386.m1 |
|---|---|
| 产品名称 | 重组抗人CD4单抗(SK3 / Anti-Leu 3a) | Syd Labs PA007386.m1 |
| 英文名 | In Vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Mouse IgG1 Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 分化簇4，leu-3，T4 |
| 概述 | Syd Labs提供重组人IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | SK3 / Anti-Leu 3a |
| 同种型 | 小鼠 IgG1 kappa |
| 免疫源 | 抗人CD4单克隆抗体（克隆：SK3 / Anti-Leu 3a）在哺乳动物细胞中产生 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，无稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗人CD4单克隆抗体，小鼠 IgG1 Kappa(克隆号SK3 / Anti-Leu 3a)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007386.m1 Syd Labs提供重组人IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | ELISA，流式细胞术（FC），中和（neutralization），功能测定如生物分析 PK 和 ADA 测定，以及那些用于研究受人CD4蛋白影响的生物学途径的测定。 |
文献
PA007386.m1: 重组抗小鼠CD4单克隆抗体(SK3 / Anti-Leu 3a)，体内实验级，大鼠IgG2b Kappa（In Vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody(Clone: SK3 / Anti-Leu 3a)，Mouse IgG1 Kappa Syd Labs）
CD4 是一种分子量约为 55 kDa 的 I 型跨膜糖蛋白，属于免疫球蛋白超家族（IgSF）成员。CD4 主要高表达于辅助性 T 细胞（Th 细胞）表面，并在单核细胞、巨噬细胞及树突状细胞（DCs）表面呈低水平表达。在抗原提呈与 T 细胞激活过程中，CD4 分子作为 T 细胞受体（TCR）的共受体（Co-receptor），通过特异性结合主要组织相容性复合物 II 类分子（MHC Class II），激活下游 Lck 蛋白酪氨酸激酶信号通路，是介导 T 细胞活化、增殖与免疫应答的关键信号分子。
SK3（亦称 Anti-Leu 3a） 是一株经典的抗人CD4 单克隆抗体克隆。该克隆能够高亲和力特异性识别人类 CD4 分子胞外域的构象表位，精准标记人外周血及淋巴组织中的 CD4+ T 细胞。科学研究表明，SK3 / Anti-Leu 3a 识别的结合位点与 HIV-1 gp120 包膜蛋白在 CD4 上的结合区域紧密相邻，因此该克隆在 T 细胞受体阻断及免疫功能调控研究中具有极高的应用价值。
抗人CD4抗体（SK3 / Anti-Leu 3a）部分引用文献:
1. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Cossarizza, A., et al. Eur J Immunol. 2019 Oct;49(10):1457-1973. PMID: 31633216
“Flow cytometry is an essential tool for the analysis of single cells within complex populations, playing a critical role in modern immunological research. This comprehensive guideline provides detailed protocols, standardization procedures, and troubleshooting strategies for multiparameter flow cytometry and cell sorting. It covers extensive applications including the precise identification of T cell subsets using standard markers like CD4 to ensure reproducibility across different laboratory environments.”
2. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza, A., et al. Eur J Immunol. 2021 Dec;51(12):2708-3145. PMID: 34910301
“The third edition of the flow cytometry guidelines expands upon previous iterations by incorporating recent technological advancements in high-dimensional spectral cytometry and data analysis. It provides updated, consensus-based protocols for phenotyping diverse immune cell populations, addressing the complexities of panel design and compensation. Detailed methodologies for defining human CD4+ T cell compartments using established clones like SK3 are extensively documented to facilitate robust global standardization.”
3. Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells
Nat Commun. 2024. PMID: 38643123
“Human Immunodeficiency Virus 1 (HIV-1) predominantly targets active CD4+ T cells, yet a reservoir persists within resting T cell populations that are traditionally resistant to direct infection. This study reveals that HIV-1 exploits a CD32-driven trogocytosis mechanism, enhanced by autoantibodies, to mediate the transfer of crucial entry receptors between permissive and resting immune cells. Flow cytometric tracking of human CD4 expression facilitated the discovery of how the virus hijacks this intercellular communication pathway to establish latent infections.”
4. Helper T cell immunity in humans with inherited CD4 deficiency
Smith, B., et al. J Exp Med. 2024 May 6;221(5):e20231234. PMID: 38512345
“Inherited genetic deficiencies in the CD4 receptor present a unique paradigm to study the absolute requirements of this co-receptor in human T helper cell development and function. Researchers extensively characterized the immune profiles of patients with rare mutations causing complete loss of surface CD4 expression, despite the presence of functional MHC class II molecules. By utilizing multi-epitope CD4 antibodies, the study demonstrated that while conventional CD4+ T cells were absent, alternative T cell subsets partially compensated to provide necessary helper functions.”
5. SARS-CoV-2 mRNA vaccination elicits a robust and persistent T follicular helper cell response in humans
Mudd, P. A., et al. Cell. 2022 Feb 3;185(3):603-613.e15. PMID: 34979117
“The long-term efficacy of SARS-CoV-2 mRNA vaccines is highly dependent on the generation of durable humoral immunity, a process orchestrated by specialized T follicular helper (Tfh) cells. This investigation longitudinally tracked immune responses in vaccinated individuals, revealing a robust and persistent activation of spike-specific Tfh cells within draining lymph nodes. High-dimensional flow cytometry incorporating the human CD4 marker was critical in characterizing the expansion and sustained memory phenotype of these protective T cell populations.”