重组抗小鼠CD3单抗(17A2) | Syd Labs PA007199.m2a
体内实验级重组抗小鼠CD3单抗（克隆号17A2，货号：PA007199.m2a），小鼠IgG2a Kappa 是用哺乳动物细胞生产的重组抗体，适用于体外和体内研究，纯度>95%。其不变区为小鼠Mouse IgG2a Kappa (mIgG2a或m2a)，可与重组小鼠IgG2a同型对照抗体配套使用。样品制备条件和样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007199.m2a |
|---|---|
| 产品名称 | 重组抗小鼠CD3单抗(17A2) | Syd Labs PA007199.m2a |
| 英文名 | In Vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody（Clone 17A2）,Mouse IgG2a Kappa |
| 说明书 | 抗小鼠CD3单抗说明书 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 分化簇3，CD3D，CD3E，CD3G |
| 克隆号 | 17A2 |
| 同种型 | 小鼠 IgG2a Kappa |
| 免疫源 | 抗小鼠CD3单克隆抗体(克隆号: 17A2)是用哺乳动物细胞生产的 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，无稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗小鼠CD3单克隆抗体(克隆号17A2)，小鼠IgG2a Kappa用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007199.m2a Syd Labs提供重组小鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | ELISA，流式细胞术（FC），中和（neutralization），功能测定如生物分析 PK 和 ADA 测定，以及那些用于研究受小鼠CD3蛋白影响的生物学途径的测定。 |
文献
PA007199.m2a：Syd Labs重组抗小鼠CD3单抗(17A2)，体内实验级，小鼠IgG2a Kappa（In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2a Kappa）
体内实验级重组抗小鼠CD3单克隆抗体（克隆号：17A2，小鼠IgG2a Kappa）是免疫学与肿瘤免疫研究中用于T细胞功能调控和体内耗竭的核心工具。CD3作为T细胞受体（TCR）复合物的重要信号转导亚基，特异性表达于小鼠成熟T细胞表面，在T细胞激活、信号传导及免疫应答中起重要作用。克隆号17A2单克隆抗体能够高亲和力结合小鼠CD3分子，广泛应用于T细胞表型分析、流式细胞术（FC）、免疫组化（IHC）及T细胞体外刺激实验。该抗体采用重组表达技术构建为小鼠IgG2a Kappa同型格式，显著提升了抗体的批间一致性与生产稳定性，同时有效降低了大鼠来源抗体在小鼠体内诱导的异源种属免疫原性风险。
该体内实验级重组抗小鼠CD3单抗（17A2，小鼠IgG2a）由哺乳动物（CHO）细胞系统重组表达生产，具备高纯度与低内毒素水平（<1 EU/mg），无叠氮化钠及无载体蛋白，专为严苛的体内（in vivo）T细胞耗竭与免疫抑制实验设计。借助小鼠IgG2a亚型对Fcγ受体（如FcγRIV）的高亲和力，重组抗小鼠CD3抗体（克隆号17A2，小鼠IgG2a）可通过有效的ADCC与CDC效应在体内实现高效且持续的T细胞清除。在自身免疫性疾病模型、器官移植排斥反应、移植物抗宿主病（GVHD）及肿瘤免疫微环境研究中，该重组单抗是高效的靶向调控工具，搭配重组小鼠IgG2a同型对照抗体配套使用有助于保障科研实验数据的准确性与高可重复性。
抗小鼠 CD3 单克隆抗体（克隆号：17A2）参考文献：
Menon, A. P., et al. Mol Ther Nucleic Acids. 2024 May;35(2):102198. PMID: 38745854
“The CD3/T cell receptor (TCR) complex is responsible for antigen-specific pathogen recognition by T cells, and initiates the signaling cascade necessary for activation of effector functions. CD3 agonistic antibodies are commonly used to expand T lymphocytes in a wide range of clinical applications, including in adoptive T cell therapy for cancer patients. […] The aptamers improved efficacy and persistence of adoptive T cell therapy by low-affinity TCR-reactive T lymphocytes in melanoma-bearing mice.”
2. Murine RAW Macrophages Are a Suitable Model to Study the CD3 Signaling in Myeloid Cells
Castro, K., et al. Cells. 2022 May 13;11(10):1635. PMID: 35626672
“In recent years, a growing body of evidence has shown the presence of a subpopulation of macrophages that express CD3, especially in the context of mycobacterial infections. […] This work aimed to evaluate the expression of CD3 in a murine macrophage cell line and its potential for the study of CD3 signaling. […] Our study provides evidence to support that RAW cells are a suitable model to study the function and signaling of the CD3 complex in myeloid cells.”
3. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza, A., Chang, H. D., Radbruch, A., et al. Eur J Immunol. 2021 Dec;51(12):2708-3145. PMID: 34910301
“The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical diagnostics and specific subsets of immune cells.”
4. Rigid crosslinking of the CD3 complex leads to superior T cell stimulation
Nelson, A. D., Wang, L., et al. Front Immunol. 2024;15:1406456. PMID: 39281668
“Functionally bivalent non-covalent Fab dimers (Bi-Fabs) specific for the TCR/CD3 complex promote CD3 signaling on T cells. While comparing functional responses to stimulation with Bi-Fab, F(ab’)2 or mAb specific for the same CD3 epitope, we observed fratricide requiring anti-CD3 clustering. […] Thus, increasing rigidity of Fab-CD3 crosslinking between opposing effector-target pairs may result in stronger T cell effector function.”
5. Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival
Shirali, A. S., et al. J Clin Invest. 2018 Nov 1;128(11):4943-4956. PMID: 30277476
“The targeted delivery of therapeutic drugs to lymph nodes (LNs) provides an unprecedented opportunity to improve the outcomes of transplantation and immune-mediated diseases. […] Here, we have synthesized a nanoparticle (NP) as a carrier of anti-CD3, and optimized the conjugation strategy to coat the NP surface with MECA79 mAb (MECA79-anti-CD3-NP) to enhance LN accumulation. […] Short-term treatment of murine cardiac allograft recipients with MECA79-anti-CD3-NP resulted in significantly prolonged allograft survival in comparison with the control groups.”