重组抗人HLA class II DR/DQ单抗(9.3F10) | Syd Labs PA007478
体内实验级重组抗人HLA class II DR/DQ单克隆抗体，小鼠IgG2a Kappa(克隆号：9.3F10，Syd Labs货号：PA007478)，适用于体外和体内研究，纯度>95%。其不变区为小鼠Mouse IgG2a Kappa (mIgG2a或m2a)，可与重组小鼠IgG2a同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007478 |
|---|---|
| 产品名称 | 重组抗人HLA class II DR/DQ单抗(9.3F10) | Syd Labs PA007478 |
| 英文名 | In vivo Grade Recombinant Anti-Human HLA class II DR/DQ Monoclonal Antibody, Mouse IgG2a Kappa (Clone: 9.3F10) |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | beta1 domain MHC class II HLA DPB, CELIAC1, class II HLA beta chain, MHC class II HLA-DR, DP, DQ |
| 概述 | Syd Labs提供重组小鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | 9.3F10 |
| 同种型 | 小鼠 IgG2a kappa |
| 应用 | ELISA、流式细胞术、中和、功能测定，如生物分析PK和ADA测定，以及用于研究受人HLA class II DR/DQ蛋白影响的生物途径的测定。 |
| 免疫源 | 抗人HLA class II DR/DQ单克隆抗体（克隆：9.3F10）在哺乳动物细胞中产生 |
| 抗体形式 | 0.2 μM过滤溶液，1x PBS |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗人HLA class II DR/DQ单克隆抗体 ，小鼠IgG2a Kappa(克隆号9.3F10)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007478 Syd Labs提供重组小鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | ELISA、流式细胞术、中和、功能测定，如生物分析PK和ADA测定，以及用于研究受人HLA class II DR/DQ蛋白影响的生物途径的测定。 |
文献
抗人HLA class II DR/DQ单克隆抗体，体内实验级重组，小鼠IgG2a Kappa(克隆号9.3F10)（In vivo Grade Recombinant Anti-Human HLA class II DR/DQ Monoclonal Antibody, Mouse IgG2a Kappa (Clone: 9.3F10)，货号：PA007478 Syd Labs）
抗人HLA class II DR/DQ单抗(9.3F10)部分引用文献:
1. Crossreactivity of a human autoimmune TCR is dominated by a single human leukocyte antigen residue
Newell, E., et al. J Clin Invest. 2014 Oct;124(10):4277-89. PMID: 25225671
“The study utilized anti-HLA-DR/DQ antibody (9.3F10) and anti-HLA-A2 (BB7.2) to assess the crossreactivity of the Ob.1A12 TCR in Jurkat cells stained with peptide/MHC tetramers and analyzed by flow cytometry. …The Ob.1A12 TCR recognized multiple self-peptides presented by HLA-DR, with a single HLA residue playing a dominant role in this crossreactivity. …In vivo experiments in HLA-DR-transgenic mice confirmed the autoimmune potential of the Ob.1A12 TCR, with TCR-transduced Jurkat cells co-cultured with antigen-presenting cells to measure activation.”
2. Self-reactive human CD4 T cell clones form unusual immunological synapses
Singh, R., et al. J Exp Med. 2012 Feb 13;209(2):335-52. PMID: 22291028
“The study used anti-HLA-DR/DQ antibody (9.3F10) and anti-HLA-DR (L243) to block MHC class II and investigate synapse formation in self-reactive CD4 T cell clones in a humanized mouse model. …Two-photon microscopy visualized synapse formation in lymph nodes, showing that 9.3F10 significantly reduced synapse stability and T cell activation. …T cell receptor signaling was assessed by measuring calcium flux upon antigen recognition.”
3. Interleukin 2-Antibody and Tumor Necrosis Factor-Antibody Fusion Proteins Induce Different Antitumor Immune Responses In Vivo
Christ, O., et al. Clin Cancer Res. 2001 May;7(5):1385-97. PMID: 11350943
“The study employed anti-HLA-DR/DQ antibody (clone 9.3F10), anti-CD80 (clone BB1), and anti-CD86 (clone IT2.2) for blocking experiments in SCID mice bearing human tumor xenografts. …IL-2 and TNF fusion proteins were administered intravenously, with tumor growth monitored over 21 days. …Flow cytometry confirmed HLA-DR/DQ expression on tumor cells, and the combination of IL-2 and TNF fusion proteins enhanced CD8+ T cell infiltration into tumors.”
4. CD4-Positive and CD8-Positive Cytotoxic T Lymphocytes Contribute to the Control of Cryptococcus neoformans in the Lungs of Mice
Kawakami, H., et al. Clin Diagn Lab Immunol. 1999 Mar;6(2):236-41. PMID: 10092809
“C57BL/6 mice were injected intraperitoneally with 0.5 mg of anti-HLA-DR/DQ mAb (9.3F10) 1 day before and 2 days after Cryptococcus neoformans infection. …The role of CD4+ and CD8+ T cells in controlling pulmonary infection was assessed, with flow cytometry quantifying T cell populations in lung tissues. …Depletion of CD4+ or CD8+ T cells increased fungal burden, demonstrating their contribution to host defense against Cryptococcus neoformans.”
5. Human Mycobacterium tuberculosis-reactive CD4+ T-cell clones from healthy, latently infected individuals
Demkowicz, A., et al. Infect Immun. 1991 Aug;59(8):2737-43. PMID: 1879230
“The study used 25 microg/mL of anti-HLA-DR/DQ antibody (9.3F10) or anti-HLA class I (W6/32) to preincubate B cells, confirming MHC class II-restricted antigen presentation. …CD4+ T cell clones from healthy individuals with latent Mycobacterium tuberculosis infection were isolated, and proliferation assays verified their specificity to mycobacterial antigens. …Flow cytometry confirmed MHC class II expression on antigen-presenting B cells, establishing that CD4+ T cell responses were restricted by HLA-DR/DQ molecules.”