重组抗人HLA-ABC单抗(W6/32) | Syd Labs PA007461
体内实验级重组抗人HLA-ABC单克隆抗体，小鼠IgG2a Kappa(克隆号W6/32，Syd Labs货号：PA007461)，适用于体外和体内研究，纯度>95%。其不变区为小鼠Mouse IgG2a Kappa (mIgG2a或m2a)，可与重组小鼠IgG2a同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007461 |
|---|---|
| 产品名称 | 重组抗人HLA-ABC单抗(W6/32) | Syd Labs PA007461 |
| 英文名 | In vivo Grade Recombinant Anti-Human HLA-ABC Monoclonal Antibody, Mouse IgG2a Kappa (Clone: W6/32) |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Major Histocompatibility Class I, MHC class I, human leukocyte antigen (HLA), HLA class I histocompatibility antigen |
| 概述 | Syd Labs提供重组小鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | W6/32 |
| 同种型 | 小鼠 IgG2a kappa |
| 应用 | ELISA、流式细胞术、中和、功能测定，如生物分析PK和ADA测定，以及用于研究受人HLA-ABC蛋白影响的生物途径的测定。 |
| 免疫源 | 抗人HLA-ABC单克隆抗体（克隆：W6/32）在哺乳动物细胞中产生 |
| 抗体形式 | 0.2 μM过滤溶液，1x PBS |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗人HLA-ABC单克隆抗体 ，小鼠IgG2a Kappa(克隆号W6/32)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007461 Syd Labs提供重组小鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | ELISA、流式细胞术、中和、功能测定，如生物分析PK和ADA测定，以及用于研究受人HLA-ABC蛋白影响的生物途径的测定。 |
文献
抗人HLA-ABC单克隆抗体，体内实验级重组，小鼠IgG2a Kappa(克隆号W6/32)（In vivo Grade Recombinant Anti-Human HLA-ABC Monoclonal Antibody, Mouse IgG2a Kappa (Clone: W6/32)，货号：PA007461 Syd Labs）
抗人HLA-ABC单抗(W6/32)部分引用文献:
1. HLA class I antibodies trigger increased adherence of monocytes to endothelial cells by eliciting an increase in endothelial P-selectin and, depending on subclass, by engaging FcγRs
Valenzuela, N. M., et al. J Immunol. 2013 Jun 15;190(12):6635-50. PMID: 23690477
“Ab-mediated rejection (AMR) of solid organ transplants is characterized by intragraft macrophages. …We investigated the mechanisms underlying monocyte recruitment by HLA class I (HLA I) Ab-activated endothelium. …Both anti-HLA I murine (m)IgG1 and mIgG2a induced endothelial P-selectin, which was required for monocyte adhesion to endothelium irrespective of subclass. …Accordingly, HLA I mIgG2a but not mIgG1 treatment of endothelial cells significantly augmented recruitment, predominantly through FcγRI, and, to a lesser extent, FcγRIIa. …Moreover, HLA I mIgG2a promoted firm adhesion of monocytes to ICAM-1 through Mac-1, which may explain the prominence of monocytes during AMR.”
2. Blockade of p-selectin is sufficient to reduce MHC I antibody-elicited monocyte recruitment in vitro and in vivo
Valenzuela, N. M., et al. Am J Transplant. 2013 Feb;13(2):299-311. PMID: 23279566
“We also determined that [Ca2+]i signaling downstream of HLA I ligation is necessary for exocytosis, consistent with previous studies. …Given the Ca2+ dependence of exocytosis, we postulated that HLA I crosslinking elicited an increase in [Ca2+]i in the endothelial cell. …Herein, we describe HLA I antibody-elicited recruitment of monocytic cells in vitro, which could be blocked by several strategies of P-selectin antagonism. …We did not observe an increase in E-selectin expression induced by HLA I crosslinking in vitro, but E-selectin may be involved in vivo. …Our results suggest that concurrent therapies may be required to inhibit the multiple mechanisms of HLA antibody-mediated graft injury.”