Syd Labs D-荧光素钾盐（CAS 115144-35-9）小鼠体内活体成像配制方法与文献应用指南
一、 Syd Labs D-荧光素钾盐标准配制方法与使用说明
针对小鼠体内活体成像实验，建议参考以下标准流程进行配制与给药：
- 储存母液配制：精准称取 D-荧光素钾盐粉末，加入无菌、无钙镁离子的 DPBS 或 PBS 缓冲液中，充分震荡溶解，配制成 15 mg/mL 的储存母液。
- 无菌过滤与保存：使用 0.22 μm 低蛋白结合滤膜 过滤除菌。按单次使用量分装，避免反复冻融，于 -20°C 或 -80°C 严格避光保存。
- 体内给药与成像参数：
- 推荐给药剂量：小鼠腹腔注射（i.p.）标准剂量为 150 mg/kg 体重（即按 10 μL/g 体重注射 15 mg/mL 母液，例如 20 g 小鼠注射 200 μL）。
- 成像时间窗口：腹腔注射后 10–15 分钟 内将小鼠置于活体成像系统（如 IVIS Lumina）中采集荧光信号。
Syd Labs D-荧光素钾盐凭借其卓越性能，已获得欧美多所大学 core facilities 的广泛好评。作为美国 Syd Labs 公司的特色产品之一，它不仅在众多欧美实验室中被广泛应用，还被许多国际知名学术期刊大量引用，这充分说明其在科研领域具有高度的实用价值。此外，美国 Syd Labs 公司还提供一系列用于体内实验的重组单抗产品，同样适用于注射体内实验，是相关科研工作者的优选试剂。
二、 学术期刊文献引用与具体应用
以下三篇高水平文献体现了 Syd Labs D-荧光素钾盐在生物科学领域的部分具体应用：
1. Engineered natural killer cells impede the immunometabolic CD73-adenosine axis in solid tumors
作者：Andrea M Chambers, Kyle B Lupo, Jiao Wang, Jingming Cao, Sagar Utturkar, Nadia Lanman, Victor Bernal-Crespo, Shadia Jalal, Sharon R Pine, Sandra Torregrosa-Allen, Bennett D Elzey, Sandro Matosevic
出处：eLife. 2022 Jul 11;11:e73699. doi: 10.7554/eLife.73699.
实验应用摘要：A549 cells (2 × 10⁶) cells were inoculated subcutaneously (SC) in the right flank of NRG mice (n=6–7/group) with 100 µL matrigel. PBS (100 µL) and engineered NK and non-engineered NK cells at 5 million cells/mouse were injected once a week via tail vein after the tumors were approximately 80 mm³. Also, 1 µg/mouse of IL-15 (Shenandoah Biotech) was injected three times per week with an intraperitoneal injection (ip). Tumor size was measured using the Spectral Ami Optical imaging system once per week. Mice were injected with 10 µL/g and 150 mg Luciferin/kg body weight with a stock solution of 15 mg/mL D-Luciferin (Syd Labs). The D-Luciferin was injected intra-peritoneally (i.p.) for 10 min before imaging. Body weights of the mice were also recorded during the treatment period. Tumor growth was monitored until mice met predefined endpoint criteria. Tumor tissues were processed in 10% neutral-buffered formalin for histological IHC analysis. For homing studies, NK cells were administered once per week with IL-2 three times per week, and NK cells were detected by IHC as described.
2. Anti-tumor antibody isotype response can be modified with locally administered immunoadjuvants
作者：Adam A Walters, Abrar Ali, Julie Tzu-Wen Wang, Khuloud T Al-Jamal
出处：Drug Deliv Transl Res. 2023 Jul;13(7):2032-2040. doi: 10.1007/s13346-022-01258-8. Epub 2022 Nov 23.
实验应用摘要：Nunc Maxisorp™ flat-bottom plates (ELISA plates) and Bicinchoninic acid assay (BCA) were obtained from Thermo Fisher Scientific (UK). The secondary antibodies were horse radish peroxidase (HRP)-conjugated Goat anti-Mouse IgM, and Goat anti-Mouse IgG1 obtained from BIO-RAD, Anti-Mouse IgG HRP-linked antibody obtained from Cell Signalling Technology (UK) and Goat Anti-Mouse IgG2c-HRP antibody obtained from SouthernBiotech (USA). Bovine serum albumin (BSA) and ovalbumin (OVA) were obtained from Sigma-Aldrich (UK). Addavax was obtained from Invivogen (France). CpG 1668 (CpG) was synthesised by Eurogentec (Belgium). Trypsin EDTA, GlutaMAX™, RPMI 1640 media, phosphate buffer saline (PBS) and fetal calf serum (FCS) were obtained from Gibco, Thermo Fischer Scientific. Tetramethylbenzidine (TMB) high-sensitivity substrate solution and ELISA stop solution were obtained from BioLegend (USA). D-Luciferin potassium salt was obtained from Syd Labs (USA).
3. Cancer immunotherapy based on image-guided STING activation by nucleotide nanocomplex-decorated ultrasound microbubbles
作者：Xuefeng Li, Sina Khorsandi, Yifan Wang, Julien Santelli, Kristin Huntoon, Nhu Nguyen, Mingming Yang, DaeYong Lee, Yifei Lu, Ruoqi Gao, Betty Y S Kim, Caroline de Gracia Lux, Robert F Mattrey, Wen Jiang, Jacques Lux
出处：Nat Nanotechnol. 2022 Aug;17(8):891-899. doi: 10.1038/s41565-022-01134-z. Epub 2022 May 30.
实验应用摘要：BALB/cJ mice were used to study the growth and metastasis of Luc-4T1 tumours in vivo as follows. At 12 days post tumour implantation, mice were intraperitoneally injected with 1.5 mg D-luciferin (Syd Labs) per 10 g body weight; at 10 min after injection, the presence and metastases of the tumours were monitored by bioluminescence imaging (BLI) using an In Vivo Imaging System (PerkinElmer IVIS® Lumina III). Data were analysed and presented with Aura Imaging software. All images were adjusted to the same color scale and display threshold. The IVIS images of the upper body were used to detect luminescence signals in the lung. After the live imaging, mice that met the criteria for early euthanasia were sacrificed, and major organs including heart, liver, spleen, lung, kidneys, and tumour were collected for additional IVIS imaging to analyse luminescence signals in these organs.
- 品牌名称：Syd Labs
- 产品名称：D-荧光素钾盐 (D-Luciferin Potassium Salt)
- 货号：MB000102-R70170 / MB102
如果需要了解更多有关 Syd Labs D-荧光素钾盐（货号：MB000102-R70170 或 MB102）的信息，如工作浓度设置、具体使用说明等资料，欢迎联系多找找获取。
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