重组抗人CD4单抗(OKT4A)流式 | Syd Labs PA007413.h1Fs
重组抗人CD4单克隆抗体，人IgG1 Fc Silent Kappa（克隆号OKT4A，Syd Labs货号：PA007413.h1Fs）是用哺乳动物细胞生产的重组抗体，适用于流式细胞术（FC）和免疫组织化学-冷冻（IHC-F）等研究，纯度>95%。其不变区为人Human IgG1 kappa (hIgG1或h1)，可与重组人IgG1同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007413.h1Fs |
|---|---|
| 产品名称 | 重组抗人CD4单抗(OKT4A)流式 | Syd Labs PA007413.h1Fs |
| 英文名 | Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4A), Human IgG1 Fc Silent Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 分化簇4，leu-3，T4 |
| 概述 | Syd Labs提供重组小鼠IgG2a同型对照抗体和重组人IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | OKT4A |
| 同种型 | 人 IgG1 kappa |
| 特异性 | 该重组 OKT4A 抗体与人和非人灵长类 CD4 特异性结合 |
| 免疫源 | 抗人CD4单克隆抗体（克隆：OKT4A）在哺乳动物细胞中产生 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，含0.09%叠氮化钠 |
| 偶联 | 非偶联 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 非偶联重组抗人CD4单克隆抗体，人IgG1 Fc Silent Kappa(克隆号OKT4A)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 12个月，保存在2 至 8°C。 请勿冻结。 |
| 注意事项 | PA007413.h1Fs Syd Labs提供重组小鼠IgG2a同型对照抗体和重组人IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | 流式细胞术（FC）和免疫组织化学-冷冻（IHC-F）。 |
文献
抗人CD4单克隆抗体(克隆号OKT4A)，非偶联重组，人IgG1 Fc Silent Kappa（Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4A), Human IgG1 Fc Silent Kappa，货号：PA007413.h1Fs Syd Labs）
重组OKT4A抗体可结合人CD4蛋白（分化群4），该蛋白是一种糖蛋白，也是T细胞受体（TCR）的共受体。CD4蛋白表达于辅助性T细胞、单核细胞、巨噬细胞和树突状细胞等免疫细胞表面。
抗人CD4单抗(OKT4A)部分参考文献:
1.The β-Chemokine Receptors CCR3 and CCR5 Facilitate Infection by Macrophage-Tropic Strain HIV-1
Doms, R. W., et al. Cell. 1998 Jun 12;93(6):1009-15. PMID: 10329570
“The OKT4A antibody (anti-human CD4, PE-conjugated) was used in flow cytometry to assess CD4 expression in cells with low CD4 levels, reducing non-specific binding and enhancing gp120 binding to CCR5. OKT4A staining confirmed CD4 dependency and inhibited CCR5-mediated fusion in a dose-dependent manner, as analyzed by flow cytometry.”
2.Restoration of Cell Surface CD4 Expression in Simian Immunodeficiency Virus-Infected Cells by Intracellular Expression of a Soluble CD4 Molecule
Turner, J. D., et al. J Virol. 1992 Nov;66(11):6802-5. PMID: 1380959
“The OKT4A antibody (anti-human CD4) was used in flow cytometry to measure CD4 surface expression in SIV-infected cells. OKT4A staining showed restored wild-type CD4 levels after soluble CD4 transfection, with controls lacking OKT4A showing no fluorescence shift, confirming prevention of CD4 down-regulation.”
3.Mapping the Paratope of Anti-CD4 Recombinant Fab 13B8.2 by Alanine Scanning Mutagenesis
Thali, M., et al. J Biol Chem. 1993 Sep 25;268(27):20785-90. PMID: 8391275
“The OKT4A antibody (anti-human CD4) was used in flow cytometry to assess binding to mutant CD4 on transfected cells. OKT4A reactivity was lost at alanine mutants (residues 58 and 62), confirming paratope overlap with 13B8.2. OKT4A served as a positive control for high-affinity CD4 binding, measured by mean fluorescence intensity.”
4.Internalization and Degradation of an Anti-CD4 Monoclonal Antibody in Human T Lymphoblasts
König, R., et al. Mol Immunol. 1993 Feb;30(3):231-9. PMID: 8468487
“The OKT4A antibody (anti-human CD4) was used as the primary probe in flow cytometry to track CD4 internalization in T lymphoblasts. Surface-bound OKT4A decreased within 30 minutes, with lysosomal inhibitors blocking degradation, confirming rapid internalization and degradation of the OKT4A-CD4 complex.”
5.Human Immunodeficiency Virus Glycoprotein (gp120) Induction of Monocyte Arachidonic Acid Metabolism
Klein, E., et al. Proc Natl Acad Sci U S A. 1989 Jan;86(2):621-5. PMID: 2536171
“The OKT4A antibody (anti-human CD4) was used in flow cytometry to confirm CD4 expression on monocytes, with 80% OKT4A positivity. OKT4A-positive cells showed increased arachidonic acid release upon gp120 stimulation, with the OKT4A epitope required for gp120 signaling, while controls lacking OKT4A showed no response.”