Syd Labs D-荧光素钾盐在活体成像中用量是多少？这些文献告诉你

D-荧光素钾盐在单只老鼠活体成像中用量是多少？Syd Labs公司的D-荧光素钾盐（D-luciferin potassium salt MB102 Syd Labs）用于表达萤火虫萤光素酶动物的活体成像分析(In Vivo Bioluminescence Imaging)，那么每只老鼠应该用多少D-荧光素钾盐?

问：单只老鼠活体成像实验，每只老鼠应该使用多少D-荧光素钾盐？

答：Syd Labs D-荧光素钾盐使用说明方法中给出建议：每次使用10 μ l的15 mg/ml荧光素原液克体重(10µl/g)。在一只重20克的老鼠中(老鼠的平均体重是在17-25克之间），对于标准的150mg /kg注射，大约是200µl。

以上是Syd Labs关于D-荧光素钾盐使用量的解答，通过使用Syd Labs D-luciferin potassium salt产品，大家还可以查看与D-荧光素钾盐相关的文献引用，这些文献都使用了D-luciferin用于成像实验:

1. “To monitor the condition of brain metastasis in the mice, bioluminescent imaging (Caliper Life Sciences, Waltham, MA, USA) was used. For this, mice were injected with D-luciferin potassium salt (SYD Labs, Inc., Natick, MA, USA) at a dose of 150 mg/kg i.p., anesthetized, and then placed into the Xenogen IVIS® spectrum imaging system (PerkinElmer, Boston, MA, USA) at 10 min after D-luciferin potassium salt injection.” Cucurbitacin E inhibits the Yes?associated protein signaling pathway and suppresses brain metastasis of human non?small cell lung cancer in a murine model.   Hsu PC, Tian B, Yang YL, Wang YC, Liu S, Urisman A, Yang CT, Xu Z, Jablons DM, You L. Oncol Rep. 2019 Aug;42(2):697-707.

2. “To assess the changes in the FLuc mRNA-LNPs over time, BALB/c mice were inoculated with FLuc mRNA-LNPs (5 μg) intravenously, intramuscularly, or subcutaneously. To detect luminescence, the mice were administered 150 mg/kg of D-luciferin (Syd labs, Hopkinton, MA, USA) intraperitoneally and anaesthetized with a mixture of oxygen and isoflurane (Wako, Osaka, Japan).” Induction of liver-resident memory T cells and protection at liver-stage malaria by mRNA-containing lipid nanoparticles Sayuri Nakamae, Satoshi Miyagawa, Koki Ogawa, Mariko Kamiya, Mayumi Taniguchi, Akari Ono, Maho Kawaguchi, Awet Alem Teklemichael, Jiun-Yu Jian, Tamasa Araki, Yukimi Katagami, Hidefumi Mukai, Takeshi Annoura, Katsuyuki Yui, Kenji Hirayama, Shigeru Kawakami, Shusaku Mizukami Front Immunol. 2023 Aug 23:14:1116299. doi: 10.3389/fimmu.2023.1116299. eCollection 2023.

3. “D-luciferin potassium salt (catalogue #MB102, Syd Labs Inc., Natick, MA) was resuspended in phosphate-buffered saline (PBS) to a final concentration of 20 mg/ml. Mice were dosed with 200 μL luciferin substrate intraperitoneally and waited for 10 minutes to allow for distribution of substrate prior to image acquisition.” Interleukin-17 promotes metastasis in an immunocompetent orthotopic mouse model of prostate cancer David Cunningham, Qiuyang Zhang, Sen Liu, Keshab R Parajuli, Qiang Nie, Lin Ma, Allen Zhang, Zhenbang Chen, Zongbing You Am J Clin Exp Urol. 2018 Jun 15;6(3):114-122. eCollection 2018.

更多的关于使用Syd Labs D-luciferin potassium salt MB102产品参考文献，请联系我们索取。

美国Syd Labs公司合成的Syd LabsD(萤）荧光素钾盐MB102产品覆盖各大学科研所和医院里的动物研究以及生物医学研究机构的科学家，已经获得广大科研工作者认可。武汉多找找科技是Syd Labs武汉地区合作推广伙伴，为科研工作者提供优质的产品与服务。Syd Labs  MB000102-R70170 or MB102 D-荧光素钾盐价格或其它更多产品参数信息，来自于武汉多找找科技，欢迎大家询单。本产品仅限于科研使用，不得用于人类或动物的诊断或治疗。

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