Syd Labs D-荧光素钾盐怎么使用？

Syd Labs D-荧光素钾盐怎么使用？这篇文献告诉你！

Syd Labs D-荧光素钾盐怎么使用？Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号MB000102-R70170或MB102-R70170），是一款由美国Syd Labs公司自主研发并长期经受国际市场考验、主要用于动物体内实验的优质产品。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）无论质量还是价格都具有很强的竞争优势，这不仅是其能够长期扎根海外市场并赢得业界广泛认可的主要原因，也是其目前进军中国市场的底气所在。

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170），多年来被众多欧美大学的core facilities购买、使用和复购，积累了良好的口碑与信誉。除了被多所欧美实验室广泛应用于动物体内实验外，Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）还反复出现在国际权威学术期刊之中，被相关科研工作者频繁收录于文献之中，这彰显了其在生命科学领域的广泛适用性和实用性。

以下文献中体现了Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170）在具体科研领域的应用：

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Shortened ex vivo manufacturing time of EGFRvIII-specific chimeric antigen receptor (CAR) T cells reduces immune exhaustion and enhances antiglioma therapeutic function

Hillary G Caruso, Ryuma Tanaka, Jiyong Liang, Xiaoyang Ling, Aria Sabbagh, Verlene K Henry, Tiara L Collier, Amy B Heimberger J Neurooncol. 2019 Dec;145(3):429-439. doi: 10.1007/s11060-019-03311-y. Epub 2019 Nov 4.

Animal experiments were carried out according to regulations from the Institutional Animal Care and Use Committee (IACUC) at MD Anderson (ACUF 00001544-RN00).Both female and male NOD.Cg-PrkdcscidIL2Rγtm1Wjl/Sz (NSG, Jackson Laboratory) mice aged 6–8 weeks were anesthetized by intraperitoneal injection using a cocktail of 10 mg/mL ketamine and 0.5 mg/mL xylazine at a dose of 0.1 mL/10 g. A guide-screw was surgically implanted 2.5 mm to the right of the coronal suture and 1 mm anterior to the bregma at a depth of 3 mm, as previously described [10]. 2 weeks after surgery, intracranial tumors were established by injection of 250,000 U87-EGFRvIII-Zeomycin fLucmKate cells in 5 μL PBS through the guide-screw. The burden of intracranial glioma was serially imaged using XenogenIVIS Spectrum (Caliper Life Sciences) 10 min after intraperitoneal injection of 3 mg D-luciferin potassium salt (cat #MB000102-R70170, syd labs). Tumor fux (photons/s/cm2/steradian) was measured using Living Image software(version 2.50, Caliper Life Sciences) in a delineated region encompassing the entire cranium. Mice were sorted into three groups to evenly distribute tumor burden based on BLI measurements 4 days after tumor injection. The mice were treated on day 5 with 4×106 T cells in 5 μL of PBS administered intracranially through the guide-screw, and treated thereafter once a week for 3 weeks. Mice were sacrifced when they displayed progressive weight loss (of>25%), rapid weight loss (of>10% within 48 h), hind limb paralysis, or any two of the following clinical symptoms of illness:ataxia, hunched posture, or irregular respiration rate.