重组仓鼠IgG1同型对照抗体 | Syd Labs PA007146
重组仓鼠IgG1同型对照抗体，体内实验级（In Vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody）。重组Armenian仓鼠IgG1同型对照抗体和Syrian仓鼠IgG1同型对照抗体（Recombinant Armenian hamster IgG1 isotype control Antibody and Syrian hamster IgG1 isotype control Antibody）均适用于体内和体外研究。与经测试的仓鼠样本的特异性结合程度低或无特异性结合。小鼠可变区和仓鼠IgG1 kappa恒定区。
产品参数
| 货号 | PA007146 |
|---|---|
| 产品名称 | 重组仓鼠IgG1同型对照抗体 | Syd Labs PA007146 |
| 英文名 | In Vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 重组仓鼠IgG1同型对照抗体，仓鼠IgG1阴性对照抗体，仓鼠IgG同型对照抗体 |
| 概述 | 重组Armenian仓鼠IgG1同型对照抗体和Syrian仓鼠IgG1同型对照抗体（Recombinant Armenian hamster IgG1 isotype control Antibody and Syrian hamster IgG1 isotype control Antibody）均适用于体内和体外研究。与经测试的仓鼠样本的特异性结合程度低或无特异性结合。小鼠可变区和仓鼠IgG1 kappa恒定区。 |
| 克隆号 | 12B9 |
| 同种型 | 仓鼠 IgG1, kappa |
| 应用 | 用于ELISA、蛋白质印迹 (WB)、流式细胞术(Flow)、免疫沉淀(IP)、免疫组织化学(石蜡)(IHC (P))、免疫组织化学(Frozen) (IHC (F))和体内动物模型研究的仓鼠IgG1 kappa抗体的同型匹配阴性对照。 |
| 免疫源 | N/A |
| 抗体形式 | 0.2 μM过滤溶液，1x PBS |
| 内毒素 | 根据 LAL 方法，≤ 1 EU每 1 mg 蛋白质。提供特级体内实验级重组仓鼠IgG1同型对照抗体（In Vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody，内毒素≤0.05 EU/mg)。 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 重组仓鼠IgG1同型对照抗体，体内实验级（In Vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody）用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 如果保存在2 至 8°C，自收到之日起可保存3个月。如果保存在-20 至 -70°C，自收到之日起可保存 12个月。 |
| 注意事项 | 重组Armenian仓鼠IgG1同型对照抗体和Syrian仓鼠IgG1同型对照抗体（Recombinant Armenian hamster IgG1 isotype control Antibody and Syrian hamster IgG1 isotype control Antibody）均适用于体内和体外研究。与经测试的仓鼠样本的特异性结合程度低或无特异性结合。小鼠可变区和仓鼠IgG1 kappa恒定区。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | 用于ELISA、蛋白质印迹 (WB)、流式细胞术(Flow)、免疫沉淀(IP)、免疫组织化学(石蜡)(IHC (P))、免疫组织化学(Frozen) (IHC (F))和体内动物模型研究的仓鼠IgG1 kappa抗体的同型匹配阴性对照。 |
文献
PA007146：重组仓鼠IgG1同型对照抗体，体内实验级（In Vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody）
仓鼠IgG抗体主要依据重链同型分为三个亚类（重链包含IgG1、IgG2、IgG3；轻链包含κ型与λ型），其中κ型是血清中含量最高的类型。IgG抗体是分子量约150 kDa的大分子，由四条肽链组成：两条分子量约50 kDa的相同重链，以及两条分子量约25 kDa的相同轻链。四条肽链依靠两条重链之间、重链与轻链之间形成的二硫键组装成Y型四聚体。
仓鼠IgG1抗体可通过Protein G磁珠进行纯化；若使用Protein A磁珠纯化，蛋白得率会相对偏低。
仓鼠IgG1同型对照抗体部分参考文献：
1.Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function.
Liu, D., et al. Nat Immunol. 2024 Jan;25(1):142-154. PMID: 38049580
“APC hamster IgG1 isotype control (anti-TNP, A19-3, 553974; 1:200) and PE hamster IgG1 isotype control (anti-TNP, A19-3, 553972; 1:200) from BD Biosciences were used for cell staining in flow cytometry experiments. Spleen marginal zone (MZ) B cells are critical for mounting antibody responses against blood-borne antigens. The study demonstrated that MZ B cells utilize adhesion G-protein-coupled receptor ADGRE5 (CD97) for retention in the spleen, and CD97 function relies on autoproteolytic cleavage and downstream signaling via Gα13 and ARHGEF1. Red blood cell expression of the CD97 ligand CD55 is essential for maintaining MZ B cell homeostasis.”
2.Reversing PD-1 Resistance in B16F10 Cells and Recovering Tumour Immunity Using a COX2 Inhibitor.
Pi, C., et al. Cancers (Basel). 2022 Aug;14(17):4134. PMID: 36077671
“PE Hamster IgG1 κ Isotype Control (553972; BD Pharmingen) was used as one of the standard isotype controls for flow cytometry analysis in tumor immunology research. Anti-PD-1/PD-L1 immunotherapy is widely applied for tumor treatment, whereas many patients develop therapeutic resistance. COX2 and prostaglandin E2 (PGE2) significantly promote malignant tumor progression. This study constructed a PD-1-resistant B16F10 tumor mouse model and confirmed that elevated COX2 expression reduces immune cell infiltration and induces acquired anti-PD-1 treatment resistance.”
3.Rare t(X;14)(q28;q32) translocation reveals link between MTCP1 and chronic lymphocytic leukemia.
Walker, J. S., et al. Nat Commun. 2021 Nov;12(1):6338. PMID: 34732719
“PE hamster IgG1 κ isotype control was used for flow cytometry immunomodulatory assessment of peripheral blood samples from Eµ-MTCP1 mice. Rare balanced translocations in cancers are often functionally unexplored. The identified t(X;14)(q28;q32) translocation colocates the MTCP1 gene with the immunoglobulin heavy chain locus, driving B-cell malignancy. Overexpression of human MTCP1 in murine B cells induces clonal CD5+/CD19+ leukemia, which recapitulates the key pathological features of human chronic lymphocytic leukemia and responds to ibrutinib targeted therapy.”
4.CTLA-4 expression by B-1a B cells is essential for immune tolerance.
Yang, Y., et al. Nat Commun. 2021 Jan;12(1):525. PMID: 33483505
“PE Hamster IgG1 isotype control (clone A19.3, BD, cat#553972) was applied as negative control in multicolor flow cytometry detection of CTLA-4 expression on B-1a cells. The study revealed that CTLA-4 expression on developmentally early B-1a cells is critical for maintaining immune self-tolerance. Loss of CTLA-4 leads to B-1a cell dysfunction, abnormal epigenetic and transcriptional activation, enhanced self-replenishment, and excessive autoimmune responses, further disrupting immune homeostasis.”
5.A protocol for the culture and isolation of murine synovial fibroblasts.
Zhao, J., et al. Biomed Rep. 2016 Aug;5(2):171-175. PMID: 27446536
“PE hamster IgG1 κ isotype control (cat. no. 553972, BD Pharmingen) was utilized for cellular marker identification and purity analysis of primary isolated murine synovial fibroblasts. Synovial fibroblast culture is an essential tool for studying synovial tissue physiology and pathology, as well as exploring therapeutic targets for chronic joint diseases such as rheumatoid arthritis. This article established a simple and reproducible protocol for isolating and culturing primary synovial fibroblasts from C57BL/6 mice and verified cell purity via vimentin, CD90.2, CD54 and CD106 markers.”